IP (Immunoprecipitation)
(Detection of human p66beta/GATAD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p66beta/GATAD2B antibody AAA211393 (lot AAA211393-2) used for IP at 6 ug per reaction. p66beta/GATAD2B was also immunoprecipitated by rabbit anti-p66beta/GATAD2B antibody AAA211393-1 For blotting immunoprecipitated p66beta/GATAD2B, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human p66beta/GATAD2B Polyclonal Antibody | anti-p66beta/GATAD2B antibody
Rabbit anti-p66beta/GATAD2B Antibody, Affinity Purified
Gene Names
GATAD2B; p68; MRD18; P66beta
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
p66beta/GATAD2B, Antibody; Rabbit anti-p66beta/GATAD2B Antibody, Affinity Purified; P66beta; transcriptional repressor p66-beta; p68; MRD18; GATA zinc finger domain-containing protein 2B; transcription repressor p66 beta component of the MeCP1 complex; p66/p68; Transcriptional repressor p66-beta; GATA zinc finger domain containing 2B; anti-p66beta/GATAD2B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-p66beta/GATAD2B antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Mouse
Immunogen
Between 543 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human p66beta/GATAD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p66beta/GATAD2B antibody AAA211393 (lot AAA211393-2) used for IP at 6 ug per reaction. p66beta/GATAD2B was also immunoprecipitated by rabbit anti-p66beta/GATAD2B antibody AAA211393-1 For blotting immunoprecipitated p66beta/GATAD2B, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human p66beta/GATAD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p66beta/GATAD2B antibody AAA211393 (lot AAA211393-2) used for IP at 6 ug per reaction. p66beta/GATAD2B was also immunoprecipitated by rabbit anti-p66beta/GATAD2B antibody AAA211393-1 For blotting immunoprecipitated p66beta/GATAD2B, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human p66beta/GATAD2B by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-p66beta/GATAD2B (Cat. No. AAA211393 Lot2) used at a dilution of 1:1000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human p66beta/GATAD2B by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-p66beta/GATAD2B (Cat. No. AAA211393 Lot2) used at a dilution of 1:1000 (1ug/ml). Detection: DAB)
Related Product Information for anti-p66beta/GATAD2B antibody
P66beta/GATAD2B interacts with MBD2 (methyl-CpG-binding domain 2) and MBD3 (methyl-CpG-binding domain 3) and participates in transcriptional repression in association with the MBD2/NuRD and MBD3/NuRD histone deacetylase complexes. P66beta/GATAD2B is SUMOylated in vivo and its repressive activity has been shown to be enhanced by SUMOylation.
Product Categories/Family for anti-p66beta/GATAD2B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,261 Da
NCBI Official Full Name
transcriptional repressor p66-beta
NCBI Official Synonym Full Names
GATA zinc finger domain containing 2B
NCBI Official Symbol
GATAD2B
NCBI Official Synonym Symbols
p68; MRD18; P66beta
NCBI Protein Information
transcriptional repressor p66-beta; p66/p68; GATA zinc finger domain-containing protein 2B; transcription repressor p66 beta component of the MeCP1 complex
UniProt Protein Name
Transcriptional repressor p66-beta
UniProt Gene Name
GATAD2B
UniProt Synonym Gene Names
KIAA1150
UniProt Entry Name
P66B_HUMAN
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Product Notes
The p66beta/GATAD2B gatad2b (Catalog #AAA211393) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-p66beta/GATAD2B Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p66beta/GATAD2B can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the p66beta/GATAD2B gatad2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p66beta/GATAD2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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