WB (Western Blot)
(Western blot analysis of p73 (Phospho-Thr86) using EGF treated NIH-3T3 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Rabbit p73 Polyclonal Antibody | anti-p73 antibody
Phospho-p73 (Thr86) Antibody
Gene Names
TP73; P73
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (88%), Bovine (100%), Sheep (100%), Dog (100%)
Applications
ELISA, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
p73, Antibody; Phospho-p73 (Thr86) Antibody; Delta N p73; DNp73; p53 like transcription factor; p53 related protein; P73; TP73; anti-p73 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (88%), Bovine (100%), Sheep (100%), Dog (100%)
Predicted Reactivity: Pig (88%), Bovine (100%), Sheep (100%), Dog (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-p73 (Thr86) Antibody detects endogenous levels of p73 only when phosphorylated at Thr86.
Tissue Specificity: Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
Tissue Specificity: Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-p73 antibody
ELISA (Peptide ELISA), WB (Western Blot)
Immunogen
A synthesized peptide derived from human p73 around the phosphorylation site of Thr86.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Phosphorylation by PLK1 and PLK3 inhibits the transcription regulator activity and pro-apoptotic function.Higher levels of phosphorylation seen in the brain from patients with Huntington disease.Polyubiquitinated by RCHY1/PIRH2; leading to its degradation by the proteasome.
Subunit Structure
Found in a complex with p53/TP53 and CABLES1. The C-terminal oligomerization domain binds to the ABL1 tyrosine kinase SH3 domain. Interacts with HECW2. Isoform Beta interacts homotypically and with p53/TP53, whereas isoform Alpha does not. Isoform Gamma interacts homotypically and with all p73 isoforms. Isoform Delta interacts with isoform Gamma, isoform Alpha, and homotypically. Isoforms Alpha and Beta interact with HIPK2. Isoform Alpha interacts with RANBP9. Isoform Beta interacts with WWOX. Interacts (via SAM domain) with FBXO45 (via B30.2/SPRY domain). Interacts with YAP1 (phosphorylated form). Interacts with HCK (via SH3 domain); this inhibits TP73 activity and degradation. (Microbial infection) Interacts with Epstein-Barr virus protein EBNA6; this interaction inhibits TP73-mediated apoptotic pathway.
Similarity
Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL1 tyrosine kinase SH3 domain.The PPxY motif mediates interaction with WWOX.Belongs to the p53 family.
Subcellular Location
Nucleus. Cytoplasm.
Note: Accumulates in the nucleus in response to DNA damage.
Note: Accumulates in the nucleus in response to DNA damage.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of p73 (Phospho-Thr86) using EGF treated NIH-3T3 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of p73 (Phospho-Thr86) using EGF treated NIH-3T3 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of extracts from Rat heart, using Phospho-p73 (Thr86) Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Rat heart, using Phospho-p73 (Thr86) Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-p73 antibody
Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
69,623 Da
NCBI Official Full Name
tumor protein p73 isoform b
NCBI Official Synonym Full Names
tumor protein p73
NCBI Official Symbol
TP73
NCBI Official Synonym Symbols
P73
NCBI Protein Information
tumor protein p73; p53-related protein; p53-like transcription factor
UniProt Protein Name
Tumor protein p73
UniProt Gene Name
TP73
UniProt Synonym Gene Names
P73
UniProt Entry Name
P73_HUMAN
Similar Products
Product Notes
The p73 tp73 (Catalog #AAA329824) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-p73 (Thr86) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (88%), Bovine (100%), Sheep (100%), Dog (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's p73 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), WB (Western Blot). Researchers should empirically determine the suitability of the p73 tp73 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p73, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.