IP (Immunoprecipitation)
(Detection of human PAF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-PAF antibody AAA212652 (lot AAA212652-1) used for IP at 6 ug per reaction. PAF was also immunoprecipitated by rabbit anti-PAF antibody BL15806. For blotting immunoprecipitated PAF, AAA212652 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human PAF Polyclonal Antibody | anti-PAF antibody
Rabbit anti-PAF Antibody, Affinity Purified
Gene Names
KIAA0101; L5; PAF; OEATC; PAF15; OEATC1; p15PAF; NS5ATP9; OEATC-1; p15/PAF; p15(PAF)
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
PAF, Antibody; Rabbit anti-PAF Antibody, Affinity Purified; HCV NS5A-transactivated protein 9; PCNA-clamp-associated factor; PCNA-associated factor of 15 kDa; p15(PAF); PCNA-associated factor; PAF15; PAF; p15PAF; p15/PAF; of 15 kDa; OEATC-1; OEATC1; OEATC; NS5ATP9; L5; hepatitis C virus NS5A-transactivated protein 9; KIAA0101; overexpressed in anaplastic thyroid carcinoma 1; PCNA clamp associated factor; anti-PAF antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PAF antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human PAF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-PAF antibody AAA212652 (lot AAA212652-1) used for IP at 6 ug per reaction. PAF was also immunoprecipitated by rabbit anti-PAF antibody BL15806. For blotting immunoprecipitated PAF, AAA212652 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PAF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-PAF antibody AAA212652 (lot AAA212652-1) used for IP at 6 ug per reaction. PAF was also immunoprecipitated by rabbit anti-PAF antibody BL15806. For blotting immunoprecipitated PAF, AAA212652 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human PAF by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-PAF (Cat. No. AAA212652 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human PAF by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-PAF (Cat. No. AAA212652 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).)
Related Product Information for anti-PAF antibody
PCNA associated factor (PAF) is a PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15004].
Product Categories/Family for anti-PAF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
7,221 Da
NCBI Official Full Name
PCNA-associated factor isoform 1
NCBI Official Synonym Full Names
KIAA0101
NCBI Official Symbol
KIAA0101
NCBI Official Synonym Symbols
L5; PAF; OEATC; PAF15; OEATC1; p15PAF; NS5ATP9; OEATC-1; p15/PAF; p15(PAF)
NCBI Protein Information
PCNA-associated factor
UniProt Protein Name
PCNA-associated factor
UniProt Gene Name
KIAA0101
UniProt Synonym Gene Names
NS5ATP9; PAF; HCV NS5A-transactivated protein 9; OEATC-1; PAF15; p15PAF
UniProt Entry Name
PAF15_HUMAN
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Product Notes
The PAF kiaa0101 (Catalog #AAA212652) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PAF Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAF can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the PAF kiaa0101 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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