Rabbit PAFAH1B1 Polyclonal Antibody | anti-PAFAH1B1 antibody

PAFAH1B1 Antibody (N-term)

Cat. Num. AAA285822

• Gene Names: PAFAH1B1; MDS; LIS1; LIS2; MDCR; PAFAH
• Reactivity: Human, mouse, rat (Predicted Reactivity: Bovine, Chicken, Monkey, Pig)
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PAFAH1B1, Antibody; PAFAH1B1 Antibody (N-term); Platelet-activating factor acetylhydrolase IB subunit alpha {ECO:0000255|HAMAP-Rule:MF_03141}; Lissencephaly-1 protein {ECO:0000255|HAMAP-Rule:MF_03141}; LIS-1 {ECO:0000255|HAMAP-Rule:MF_03141}; PAF acetylhydrolase 45 kDa subunit {ECO:0000255|HAMAP-Rule:MF_03141}; PAF-AH 45 kDa subunit {ECO:0000255|HAMAP-Rule:MF_03141}; PAF-AH alpha {ECO:0000255|HAMAP-Rule:MF_03141}; PAFAH alpha {ECO:0000255|HAMAP-Rule:MF_03141}; PAFAH1B1 {ECO:0000255|HAMAP-Rule:MF_03141}; anti-PAFAH1B1 antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat (Predicted Reactivity: Bovine, Chicken, Monkey, Pig)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PAFAH1B1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 76-110 amino acids from the N-terminal region of human PAFAH1B1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 410

• Applicable Applications for anti-PAFAH1B1 antibody: WB (Western Blot), IHC (Immunohistochemistry), ELISA
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of lysates from HeLa, mouse C2C12, rat C6 cell line (from left to right), using PAFAH1B1 Antibody (N-term). AAA285822 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded H. brain section using PAFAH1B1 Antibody (N-term). AAA285822 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

Related Product Information for anti-PAFAH1B1 antibody

Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet- activating factor (PAF) by removing the acetyl group at the SN-2 position (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing.

Product Categories/Family for anti-PAFAH1B1 antibody

Crown Antibodies; Neuroscience

References

Reiner O.,et al.Nature 364:717-721(1993).
Lo Nigro C.,et al.Hum. Mol. Genet. 6:157-164(1997).
Zhao M.J.,et al.Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
Feng Z.,et al.Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4557741
• NCBI GeneID: 5048
• NCBI Accession #: NP_000421.1
• NCBI GenBank Nucleotide #: NM_000430.3
• UniProt Accession #: P43034; Q8WZ88; Q8WZ89; B2R7Q7
• Molecular Weight: 46638
• NCBI Official Full Name: platelet-activating factor acetylhydrolase IB subunit alpha
• NCBI Official Synonym Full Names: platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
• NCBI Official Symbol: PAFAH1B1
• NCBI Official Synonym Symbols: MDS; LIS1; LIS2; MDCR; PAFAH
• NCBI Protein Information: platelet-activating factor acetylhydrolase IB subunit alpha
• UniProt Protein Name: Platelet-activating factor acetylhydrolase IB subunit alpha
• UniProt Gene Name: PAFAH1B1
• UniProt Synonym Gene Names: LIS-1; PAF-AH 45 kDa subunit; PAFAH alpha
• UniProt Entry Name: LIS1_HUMAN