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product-image-AAA52516_WB10.jpg WB (Western Blot) (Mouse Pak4 Antibody (Center) Western blot of Neuro-2a cell line lysates (35 ug/lane). This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).)

Rabbit anti-Human, Mouse PAK4 Polyclonal Antibody | anti-PAK4 antibody

Rabbit Polyclonal to Mouse PAK4

Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
PAK4, Antibody; Rabbit Polyclonal to Mouse PAK4; Anti-PAK4 Antibody (aa186-206) IHC-plus; PAK4; KIAA1142; p21(CDKN1A)-activated kinase 4; PAK-4; p21-activated kinase 4; Mouse PAK4; anti-PAK4 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Mouse PAK4
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.09% sodium azide
Sequence Length
591
Applicable Applications for anti-PAK4 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry)
Target Species
Mouse
Immunogen Description
KLH-conjugated synthetic peptide from internal region of Mouse Pak4.
Immunogen Type
Synthetic peptide
Immunogen
PAK4 antibody was raised against kLH-conjugated synthetic peptide from internal region of Mouse Pak4.
Antigen Modification
aa186-206
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

WB (Western Blot)

(Mouse Pak4 Antibody (Center) Western blot of Neuro-2a cell line lysates (35 ug/lane). This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).)

product-image-AAA52516_WB10.jpg WB (Western Blot) (Mouse Pak4 Antibody (Center) Western blot of Neuro-2a cell line lysates (35 ug/lane). This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).)

WB (Western Blot)

(Mouse Pak4 Antibody (Center) Western blot of MCF-7 cell line lysates (35 ug/lane). This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).)

product-image-AAA52516_WB11.jpg WB (Western Blot) (Mouse Pak4 Antibody (Center) Western blot of MCF-7 cell line lysates (35 ug/lane). This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).)

FCM/FACS (Flow Cytometry)

(Mouse Pak4 Antibody (Center) flow cytometry of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA52516_FCM13.jpg FCM/FACS (Flow Cytometry) (Mouse Pak4 Antibody (Center) flow cytometry of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry)

(Anti-PAK4 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

product-image-AAA52516_IHC15.jpg IHC (Immunohistochemistry) (Anti-PAK4 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Related Product Information for anti-PAK4 antibody
PAK4, a PAK (STE20) type protein kinase, functions in reorganization of the actin cytoskeleton, filipodia formation and cell migration. The protein has been shown to interact with the GTP-bound form of Cdc42 and weakly with RAC and to be stimulated by HGF via phosphoinositide 3-kinase (PI3K). Activated PAK4 has been shown to activate the c-Jun N-terminal kinase pathway.
Product Categories/Family for anti-PAK4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,940 Da
NCBI Official Full Name
serine/threonine-protein kinase PAK 4 isoform 1
NCBI Official Synonym Full Names
p21 protein (Cdc42/Rac)-activated kinase 4
NCBI Official Symbol
PAK4
NCBI Protein Information
serine/threonine-protein kinase PAK 4; PAK-4; p21-activated kinase 4; p21(CDKN1A)-activated kinase 4; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
UniProt Protein Name
Serine/threonine-protein kinase PAK 4
UniProt Gene Name
PAK4
UniProt Synonym Gene Names
KIAA1142; PAK-4
UniProt Entry Name
PAK4_HUMAN

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Product Notes

The PAK4 pak4 (Catalog #AAA52516) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Mouse PAK4 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PAK4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the PAK4 pak4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAK4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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