WB (Western Blot)
(Detection of human PAN3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PAN3 antibody AAA213006 (lot AAA213006-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human PAN3 Polyclonal Antibody | anti-PAN3 antibody
Rabbit anti-PAN3 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PAN3, Antibody; Rabbit anti-PAN3 Antibody, Affinity Purified; PAN3 poly(A) specific ribonuclease subunit homolog; poly(A)-nuclease deadenylation complex subunit 3; PAN2-PAN3 deadenylation complex subunit PAN3; PAN deadenylation complex subunit 3; PABP-dependent poly(A) nuclease 3; PAB-dependent poly(A)-specific ribonuclease subunit PAN3; PAB-dependent poly(A)-specific ribonuclease subunit 3; PAB1P-dependent poly(A)-specific ribonuclease; PAB1P-dependent poly(A)-nuclease; PABP1-dependent poly A-specific ribonuclease subunit PAN3; poly(A) specific ribonuclease subunit PAN3; anti-PAN3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PAN3 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 175 and 225
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PAN3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PAN3 antibody AAA213006 (lot AAA213006-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human PAN3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PAN3 antibody AAA213006 (lot AAA213006-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PAN3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PAN3 antibody AAA213006 (lot AAA213006-1) used for IP at 6 ug per reaction. PAN3 was also immunoprecipitated by rabbit anti-PAN3 antibody BL19308. For blotting immunoprecipitated PAN3, AAA213006 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PAN3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PAN3 antibody AAA213006 (lot AAA213006-1) used for IP at 6 ug per reaction. PAN3 was also immunoprecipitated by rabbit anti-PAN3 antibody BL19308. For blotting immunoprecipitated PAN3, AAA213006 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-PAN3 antibody
PAB-dependent poly(A)-specific ribonuclease subunit PAN3 is a regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by poly(A)-binding protein (PABP), which is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with PABP and to miRNA targets via its interaction with GW182 family proteins [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q58A45].
Product Categories/Family for anti-PAN3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
72,933 Da
NCBI Official Full Name
PAB-dependent poly(A)-specific ribonuclease subunit PAN3
NCBI Official Synonym Full Names
PAN3 poly(A) specific ribonuclease subunit
NCBI Official Symbol
PAN3
NCBI Protein Information
PAB-dependent poly(A)-specific ribonuclease subunit PAN3; PAB-dependent poly(A)-specific ribonuclease subunit PAN3; PAB1P-dependent poly(A)-nuclease; PABP-dependent poly(A) nuclease 3; PAN deadenylation complex subunit 3; PAN3 poly(A) specific ribonucleas
UniProt Protein Name
PAB-dependent poly(A)-specific ribonuclease subunit PAN3
UniProt Gene Name
PAN3
UniProt Entry Name
PAN3_HUMAN
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Product Notes
The PAN3 pan3 (Catalog #AAA213006) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PAN3 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAN3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PAN3 pan3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAN3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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