WB (Western Blot)
(PARP-1 N-terminal antibody (pAb) tested by Western blot. HeLa nuclear extract (25 ug) was probed with PARP-1 N-terminal antibody at a dilution of 1 ug/ml.)
Rabbit anti-Human PARP-1 Polyclonal Antibody | anti-PARP-1 antibody
PARP-1 N-terminal antibody (pAb)
Gene Names
PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
PARP-1, Antibody; PARP-1 N-terminal antibody (pAb); PARP; PPOL; ADPRT; ARTD1; ADPRT1; ADPRT 1; pADPRT-1; anti-PARP-1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a sera version of this antibody is also available.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-PARP-1 antibody
WB (Western Blot), IP (Immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This antibody was raised against a His-Tagged fusion protein corresponding to the N-terminal half of human PARP-1.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(PARP-1 N-terminal antibody (pAb) tested by Western blot. HeLa nuclear extract (25 ug) was probed with PARP-1 N-terminal antibody at a dilution of 1 ug/ml.)
WB (Western Blot)
(PARP-1 N-terminal antibody (pAb) tested by Western blot. HeLa nuclear extract (25 ug) was probed with PARP-1 N-terminal antibody at a dilution of 1 ug/ml.)
WB (Western Blot)
(PARP-1 N-terminal antibody (pAb) tested by Immunoprecipitation. 10 ug of PARP-1 antibody was used to immunoprecipitate PARP-1 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ug of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the PARP-1 antibody at a dilution of 1 ug/ml.)
WB (Western Blot)
(PARP-1 N-terminal antibody (pAb) tested by Immunoprecipitation. 10 ug of PARP-1 antibody was used to immunoprecipitate PARP-1 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ug of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the PARP-1 antibody at a dilution of 1 ug/ml.)
ChIP (Chromatin Immunoprecipitation)
(PARP-1 N-terminal antibody (pAb) antibody (rAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human NCI-H209 lung cancer cells and 5 ug of PARP-1 N-terminal antibody (pAb). ChIP DNA was sequenced on the Illumina NextSeq and 7.7 million sequence tags were mapped to identify PARP binding sites on chromosome 2.)
ChIP (Chromatin Immunoprecipitation)
(PARP-1 N-terminal antibody (pAb) antibody (rAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human NCI-H209 lung cancer cells and 5 ug of PARP-1 N-terminal antibody (pAb). ChIP DNA was sequenced on the Illumina NextSeq and 7.7 million sequence tags were mapped to identify PARP binding sites on chromosome 2.)
Related Product Information for anti-PARP-1 antibody
Short Description: Our PARP-1 N-terminal antibody (pAb) was raised in a Rabbit host. It has been validated for use in ChIP-Seq, Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: PARP-1 N-terminal (ADPRT) encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. Cleavage of PARP-1 (ADPRT) occurs following caspase activation during apoptosis. For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section of our website.
Background: PARP-1 N-terminal (ADPRT) encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. Cleavage of PARP-1 (ADPRT) occurs following caspase activation during apoptosis. For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section of our website.
Product Categories/Family for anti-PARP-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
113,084 Da
NCBI Official Full Name
poly
NCBI Official Synonym Full Names
poly (ADP-ribose) polymerase 1
NCBI Official Symbol
PARP1
NCBI Official Synonym Symbols
PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
NCBI Protein Information
poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase NAD(+); ADP-ribosyltransferase diphtheria toxin-like 1; NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose)
UniProt Protein Name
Poly [ADP-ribose] polymerase 1
UniProt Gene Name
PARP1
UniProt Synonym Gene Names
ADPRT; PPOL; PARP-1; ARTD1; ADPRT 1
UniProt Entry Name
PARP1_HUMAN
Similar Products
Product Notes
The PARP-1 parp1 (Catalog #AAA60065) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PARP-1 N-terminal antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PARP-1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the PARP-1 parp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARP-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.