IHC (Immunohistochemisry)
(IHC image of AAA234972 diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Rabbit anti-Human PARP3 Polyclonal Antibody | anti-PARP3 antibody
PARP3 Antibody
Gene Names
PARP3; IRT1; ARTD3; ADPRT3; ADPRTL2; ADPRTL3; PADPRT-3
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
PARP3, Antibody; PARP3 Antibody; Poly [ADP-ribose] polymerase 3; PARP-3; hPARP-3; ADP-ribosyltransferase diphtheria toxin-like 3; ARTD3; IRT1; NAD(+) ADP-ribosyltransferase 3; ADPRT-3; Poly[ADP-ribose] synthase 3; pADPRT-3; PARP3; ADPRT3; ADPRTL3; anti-PARP3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
540
Applicable Applications for anti-PARP3 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant Human Poly [ADP-ribose] polymerase 3 protein (1-240AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IHC (Immunohistochemisry)
(IHC image of AAA234972 diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemisry)
(IHC image of AAA234972 diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohiostchemistry)
(IHC image of AAA234972 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohiostchemistry)
(IHC image of AAA234972 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot)
(Western BlotPositive WB detected in: A549 whole cell lysate, LO2 whole cell lysateAll lanes: PARP3 antibody at 3.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 61 KDaObserved band size: 61 KDa)
WB (Western Blot)
(Western BlotPositive WB detected in: A549 whole cell lysate, LO2 whole cell lysateAll lanes: PARP3 antibody at 3.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 61 KDaObserved band size: 61 KDa)
Related Product Information for anti-PARP3 antibody
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Negatively influences the G1/S cell cycle progression without interfering with centrosome duplication. Binds DNA. May be involved in the regulation of PRC2 and PRC3 complex-dependent gene silencing.
Product Categories/Family for anti-PARP3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,865 Da
NCBI Official Full Name
poly
NCBI Official Synonym Full Names
poly(ADP-ribose) polymerase family member 3
NCBI Official Symbol
PARP3
NCBI Official Synonym Symbols
IRT1; ARTD3; ADPRT3; ADPRTL2; ADPRTL3; PADPRT-3
NCBI Protein Information
poly [ADP-ribose] polymerase 3
UniProt Protein Name
Poly [ADP-ribose] polymerase 3
UniProt Gene Name
PARP3
UniProt Synonym Gene Names
ADPRT3; ADPRTL3; PARP-3; hPARP-3; ARTD3; ADPRT-3; pADPRT-3
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Product Notes
The PARP3 parp3 (Catalog #AAA234972) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PARP3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PARP3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PARP3 parp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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