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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of widr cells using PAX4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit PAX4 Polyclonal Antibody | anti-PAX4 antibody
PAX4 Antibody (Center)
Gene Names
PAX4; KPD; MODY9
Reactivity
Human, rat (Predicted Reactivity: Mouse)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PAX4, Antibody; PAX4 Antibody (Center); Paired box protein Pax-4; PAX4; anti-PAX4 antibody
Host
Rabbit
Reactivity
Human, rat (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PAX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the Central region of human PAX4.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
171-200
Sequence Length
343
Applicable Applications for anti-PAX4 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Cellular Location
Nucleus.
Function
Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that binds to a common element in the glucagon, insulin and somatostatin promoters. Competes with PAX6 for this same promoter binding site. Isoform 2 appears to be a dominant negative form antagonizing PAX4 transcriptional activity.
Precautions
PAX4 Antibody (Center) is for research use only.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of widr cells using PAX4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of widr cells using PAX4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PAX4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PAX4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of PAX4 antibody (Center) (RB20216) in CEM cell line lysates (35ug/lane). PAX4 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of PAX4 antibody (Center) (RB20216) in CEM cell line lysates (35ug/lane). PAX4 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(All lanes : Anti-PAX4 Antibody (Center) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: rat liver lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-PAX4 Antibody (Center) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: rat liver lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-PAX4 antibody
PAX4 is a member of the paired box (PAX) family of transcription factors. These proteins play critical roles during fetal development and cancer growth. The paired box 4 gene is involved in pancreatic islet development and mouse studies have demonstrated a role for this gene in differentiation of insulin-producing beta cells.
Product Categories/Family for anti-PAX4 antibody
References
Hata,S., Int. J. Oncol. 33 (5), 1065-1071 (2008)
Zhang,Y., Diabetes Res. Clin. Pract. 81 (3), 365-369 (2008)
Zhang,Y., Diabetes Res. Clin. Pract. 81 (3), 365-369 (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37833
NCBI Official Full Name
paired box protein Pax-4
NCBI Official Synonym Full Names
paired box 4
NCBI Official Symbol
PAX4
NCBI Official Synonym Symbols
KPD; MODY9
NCBI Protein Information
paired box protein Pax-4
UniProt Protein Name
Paired box protein Pax-4
UniProt Gene Name
PAX4
UniProt Entry Name
PAX4_HUMAN
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Product Notes
The PAX4 pax4 (Catalog #AAA287690) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 171-200. The PAX4 Antibody (Center) reacts with Human, rat (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's PAX4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PAX4 pax4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAX4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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