IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using Paxillin(phospho-Tyr88) Antibody.)
Rabbit anti-Human Paxillin Polyclonal Antibody | anti-PXN antibody
Paxillin (phospho-Tyr88) Antibody
Reactivity
Human
Applications
Immunofluorescence, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Synonyms
Paxillin, Antibody; Paxillin (phospho-Tyr88) Antibody; PAXI; PXN; anti-PXN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 88.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
591
Applicable Applications for anti-PXN antibody
IF (Immunofluorescence), WB (Western Blot)
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of tyrosine 88 (P-V-Y(p)-G-S) derived from Human Paxillin.
Modification
Phospho-Tyr88
Target Name
Paxillin
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using Paxillin(phospho-Tyr88) Antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed Hela cells using Paxillin(phospho-Tyr88) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Paxillin(phospho-Tyr88) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Paxillin(phospho-Tyr88) Antibody.)
Related Product Information for anti-PXN antibody
An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions
Product Categories/Family for anti-PXN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,604 Da
NCBI Official Full Name
paxillin isoform 2
NCBI Official Synonym Full Names
paxillin
NCBI Official Symbol
PXN
NCBI Protein Information
paxillin
UniProt Protein Name
Paxillin
UniProt Gene Name
PXN
UniProt Entry Name
PAXI_HUMAN
Similar Products
Product Notes
The PXN pxn (Catalog #AAA304217) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Paxillin (phospho-Tyr88) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Paxillin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the PXN pxn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Paxillin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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