IHC (Immunohiostchemistry)
(Anti-PBP antibody, AAA45030, IHC(P)IHC(P): Rat Kidney Tissue)
Rabbit anti-Mouse, Rat PBP Polyclonal Antibody | anti-PEBP1 antibody
Anti-PBP antibody
Gene Names
Pebp1; Pbp; HCNP; Pbp1; Pbpr; Rkip
Reactivity
Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
PBP, Antibody; Anti-PBP antibody; Phosphatidylethanolamine-binding protein 1(PEBP-1); phosphatidylethanolamine binding protein 1; HCNP antibody; HCNPpp antibody; Hippocampal cholinergic neurostimulating peptide antibody; Neuropolypeptide h3 antibody; PEBP antibody; PEBP 1 antibody; PEBP-1 antibody; PEBP1 antibody; PEBP1_HUMAN antibody; Phosphatidylethanolamine binding protein antibody; Phosphatidylethanolamine binding protein 1 antibody; Prostatic binding protein antibody; Prostatic-binding protein antibody; R kip antibody; Raf kinase inhibitor protein antibody; Raf kinase inhibitory protein antibody; RKIP antibody; anti-PEBP1 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
187
Applicable Applications for anti-PEBP1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse PBP (15-29aa QEVDEPPQHALRVDY), identical to the related rat sequence.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Anti-PBP antibody, AAA45030, IHC(P)IHC(P): Rat Kidney Tissue)
IHC (Immunohiostchemistry)
(Anti-PBP antibody, AAA45030, IHC(P)IHC(P): Rat Kidney Tissue)
WB (Western Blot)
(Anti-PBP antibody, AAA45030, Western blottingAll lanes: Anti PBP (AAA45030) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Lung Tissue Lysate at 50ugLane 3: Rat Liver Tissue Lysate at 50ugPredicted bind size: 21KDObserved bind size: 21KD)
WB (Western Blot)
(Anti-PBP antibody, AAA45030, Western blottingAll lanes: Anti PBP (AAA45030) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Lung Tissue Lysate at 50ugLane 3: Rat Liver Tissue Lysate at 50ugPredicted bind size: 21KDObserved bind size: 21KD)
Related Product Information for anti-PEBP1 antibody
Description: Rabbit IgG polyclonal antibody for Phosphatidylethanolamine-binding protein 1(PEBP1) detection. Tested with WB, IHC-P in Mouse, Rat.
Background: PEBP1(Phosphatidylethanolamine-binding protein 1), also called PBP, RKIP, inhibits the phosphorylation and activation of MEK by RAF1. PEBP1 is identical to the phosphatidylethanolamine-binding protein(PBP) with a relative molecular mass of 23 kD. The PEBP1 gene is mapped on 12q24.23. PEBP1 coimmunoprecipitates with RAF1 and MEK from cell lysates and colocalizes with RAF1 when examined by confocal microscopy. PEBP1 overexpression interferes with the activation of MEK and ERK, induction of AP1-dependent reporter genes, and transformation elicited by an oncogenically activated RAF1 kinase. PEBP1 expression was rapidly upregulated during induction of chemotherapy-triggered apoptosis in human prostate and breast cancer cell lines, and maximal RKIP expression correlated perfectly with the onset of apoptosis by Chatterjee et al(2004). RKIP depletion decreased the mitotic index, the number of metaphase cells, traversal times from nuclear envelope breakdown to anaphase, and an override of mitotic checkpoints induced by spindle poisons.
Background: PEBP1(Phosphatidylethanolamine-binding protein 1), also called PBP, RKIP, inhibits the phosphorylation and activation of MEK by RAF1. PEBP1 is identical to the phosphatidylethanolamine-binding protein(PBP) with a relative molecular mass of 23 kD. The PEBP1 gene is mapped on 12q24.23. PEBP1 coimmunoprecipitates with RAF1 and MEK from cell lysates and colocalizes with RAF1 when examined by confocal microscopy. PEBP1 overexpression interferes with the activation of MEK and ERK, induction of AP1-dependent reporter genes, and transformation elicited by an oncogenically activated RAF1 kinase. PEBP1 expression was rapidly upregulated during induction of chemotherapy-triggered apoptosis in human prostate and breast cancer cell lines, and maximal RKIP expression correlated perfectly with the onset of apoptosis by Chatterjee et al(2004). RKIP depletion decreased the mitotic index, the number of metaphase cells, traversal times from nuclear envelope breakdown to anaphase, and an override of mitotic checkpoints induced by spindle poisons.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
20,830 Da
NCBI Official Full Name
Phosphatidylethanolamine-binding protein 1
NCBI Official Synonym Full Names
phosphatidylethanolamine binding protein 1
NCBI Official Symbol
Pebp1
NCBI Official Synonym Symbols
Pbp; HCNP; Pbp1; Pbpr; Rkip
NCBI Protein Information
phosphatidylethanolamine-binding protein 1; HCNPpp; PEBP-1; Raf-1 inhibitor protein; Raf-1 kinase inhibitor protein
UniProt Protein Name
Phosphatidylethanolamine-binding protein 1
UniProt Gene Name
Pebp1
UniProt Synonym Gene Names
Pbp; Pebp; PEBP-1; HCNP
UniProt Entry Name
PEBP1_MOUSE
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Product Notes
The PEBP1 pebp1 (Catalog #AAA45030) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PBP antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PBP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PEBP1 pebp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PBP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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