IHC (Immunohiostchemistry)
(PCDHA3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHA3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human PCDHA3 Polyclonal Antibody | anti-PCDHA3 antibody
PCDHA3 Antibody (C-term)
Gene Names
PCDHA3; PCDH-ALPHA3
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PCDHA3, Antibody; PCDHA3 Antibody (C-term); Protocadherin alpha-3; PCDH-alpha-3; PCDHA3; anti-PCDHA3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PCDHA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 773-800 amino acids from the C-terminal region of human PCDHA3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
773-800
Sequence Length
950
Applicable Applications for anti-PCDHA3 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(PCDHA3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHA3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(PCDHA3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHA3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(PCDHA3 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the PCDHA3 antibody detected the PCDHA3 protein (arrow).)
WB (Western Blot)
(PCDHA3 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the PCDHA3 antibody detected the PCDHA3 protein (arrow).)
Related Product Information for anti-PCDHA3 antibody
This gene is a member of the protocadherin alpha gene
cluster, one of three related gene clusters tandemly linked on
chromosome five that demonstrate an unusual genomic organization
similar to that of B-cell and T-cell receptor gene clusters. The
alpha gene cluster is composed of 15 cadherin superfamily genes
related to the mouse CNR genes and consists of 13 highly similar
and 2 more distantly related coding sequences. The tandem array of
15 N-terminal exons, or variable exons, are followed by downstream
C-terminal exons, or constant exons, which are shared by all genes
in the cluster. The large, uninterrupted N-terminal exons each
encode six cadherin ectodomains while the C-terminal exons encode
the cytoplasmic domain. These neural cadherin-like cell adhesion
proteins are integral plasma membrane proteins that most likely
play a critical role in the establishment and function of specific
cell-cell connections in the brain. Alternative splicing has been
observed and additional variants have been suggested but their
full-length nature has yet to be determined.
cluster, one of three related gene clusters tandemly linked on
chromosome five that demonstrate an unusual genomic organization
similar to that of B-cell and T-cell receptor gene clusters. The
alpha gene cluster is composed of 15 cadherin superfamily genes
related to the mouse CNR genes and consists of 13 highly similar
and 2 more distantly related coding sequences. The tandem array of
15 N-terminal exons, or variable exons, are followed by downstream
C-terminal exons, or constant exons, which are shared by all genes
in the cluster. The large, uninterrupted N-terminal exons each
encode six cadherin ectodomains while the C-terminal exons encode
the cytoplasmic domain. These neural cadherin-like cell adhesion
proteins are integral plasma membrane proteins that most likely
play a critical role in the establishment and function of specific
cell-cell connections in the brain. Alternative splicing has been
observed and additional variants have been suggested but their
full-length nature has yet to be determined.
Product Categories/Family for anti-PCDHA3 antibody
References
Gregorio, S.P., et al. Psychiatry Res 165 (1-2), 1-9 (2009) :
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Wu, Q., et al. Genome Res. 11(3):389-404(2001)
Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Wu, Q., et al. Genome Res. 11(3):389-404(2001)
Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
102428
NCBI Official Full Name
protocadherin alpha-3 isoform 1
NCBI Official Synonym Full Names
protocadherin alpha 3
NCBI Official Symbol
PCDHA3
NCBI Official Synonym Symbols
PCDH-ALPHA3
NCBI Protein Information
protocadherin alpha-3
UniProt Protein Name
Protocadherin alpha-3
UniProt Gene Name
PCDHA3
UniProt Synonym Gene Names
PCDH-alpha-3
UniProt Entry Name
PCDA3_HUMAN
Similar Products
Product Notes
The PCDHA3 pcdha3 (Catalog #AAA284179) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 773-800. The PCDHA3 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCDHA3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PCDHA3 pcdha3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCDHA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.