FCM/FACS (Flow Cytometry)
(PCDHGA3 Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human PCDHGA3 Polyclonal Antibody | anti-PCDHGA3 antibody
PCDHGA3 Antibody (Center)
Gene Names
PCDHGA3; PCDH-GAMMA-A3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PCDHGA3, Antibody; PCDHGA3 Antibody (Center); Protocadherin gamma-A3; PCDH-gamma-A3; PCDHGA3; anti-PCDHGA3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PCDHGA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284-311 amino acids from the Central region of human PCDHGA3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
284-311
Sequence Length
829
Applicable Applications for anti-PCDHGA3 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(PCDHGA3 Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PCDHGA3 Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(PCDHGA3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHGA3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(PCDHGA3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHGA3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(PCDHGA3 Antibody (Center) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the PCDHGA3 antibody detected the PCDHGA3 protein (arrow).)
WB (Western Blot)
(PCDHGA3 Antibody (Center) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the PCDHGA3 antibody detected the PCDHGA3 protein (arrow).)
Related Product Information for anti-PCDHGA3 antibody
This gene is a member of the protocadherin gamma gene
cluster, one of three related clusters tandemly linked on
chromosome five. These gene clusters have an immunoglobulin-like
organization, suggesting that a novel mechanism may be involved in
their regulation and expression. The gamma gene cluster includes 22
genes divided into 3 subfamilies. Subfamily A contains 12 genes,
subfamily B contains 7 genes and 2 pseudogenes, and the more
distantly related subfamily C contains 3 genes. The tandem array of
22 large, variable region exons are followed by a constant region,
containing 3 exons shared by all genes in the cluster. Each
variable region exon encodes the extracellular region, which
includes 6 cadherin ectodomains and a transmembrane region. The
constant region exons encode the common cytoplasmic region. These
neural cadherin-like cell adhesion proteins most likely play a
critical role in the establishment and function of specific
cell-cell connections in the brain. Alternative splicing has been
described for the gamma cluster genes.
cluster, one of three related clusters tandemly linked on
chromosome five. These gene clusters have an immunoglobulin-like
organization, suggesting that a novel mechanism may be involved in
their regulation and expression. The gamma gene cluster includes 22
genes divided into 3 subfamilies. Subfamily A contains 12 genes,
subfamily B contains 7 genes and 2 pseudogenes, and the more
distantly related subfamily C contains 3 genes. The tandem array of
22 large, variable region exons are followed by a constant region,
containing 3 exons shared by all genes in the cluster. Each
variable region exon encodes the extracellular region, which
includes 6 cadherin ectodomains and a transmembrane region. The
constant region exons encode the common cytoplasmic region. These
neural cadherin-like cell adhesion proteins most likely play a
critical role in the establishment and function of specific
cell-cell connections in the brain. Alternative splicing has been
described for the gamma cluster genes.
Product Categories/Family for anti-PCDHGA3 antibody
References
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Wu, Q., et al. Genome Res. 11(3):389-404(2001)
Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)
Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000)
Wu, Q., et al. Genome Res. 11(3):389-404(2001)
Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)
Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
100971
NCBI Official Full Name
protocadherin gamma-A3 isoform 1
NCBI Official Synonym Full Names
protocadherin gamma subfamily A, 3
NCBI Official Symbol
PCDHGA3
NCBI Official Synonym Symbols
PCDH-GAMMA-A3
NCBI Protein Information
protocadherin gamma-A3
UniProt Protein Name
Protocadherin gamma-A3
UniProt Gene Name
PCDHGA3
UniProt Synonym Gene Names
PCDH-gamma-A3
UniProt Entry Name
PCDG3_HUMAN
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Product Notes
The PCDHGA3 pcdhga3 (Catalog #AAA284336) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 284-311. The PCDHGA3 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCDHGA3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PCDHGA3 pcdhga3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCDHGA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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