IHC (Immunohiostchemistry)
(Immunochemical staining of human PCMT1 in human stomach with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human PCMT1 Polyclonal Antibody | anti-PCMT1 antibody
Anti-PCMT1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PCMT1, Antibody; Anti-PCMT1 Antibody, Rabbit Polyclonal; PCMT1 Antibody, Rabbit PAb, Antigen Affinity Purified; protein-L-isoaspartate (D-aspartate) O-methyltransferase; Anti-PIMT Antibody; anti-PCMT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PCMT1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PCMT1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human PCMT1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PCMT1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Immunochemical staining of human PCMT1 in human stomach with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human PCMT1 in human stomach with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-PCMT1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:28 kDa)
WB (Western Blot)
(Anti-PCMT1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:28 kDa)
Related Product Information for anti-PCMT1 antibody
Protein L-isoaspartate (D-aspartate) O-methyltransferase (PIMT) repairs modified proteins and is encoded by PCMT1, located in a region linked to type 1 diabetes (T1D), namely IDDM5. L-Isoaspartyl (D-aspartyl) O-methyltransferase (PCMT1) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form. In the course of this reaction, PCMT1 converts the methyl donor S-adenosylmethionine (AdoMet) to S-adenosylhomocysteine (AdoHcy). A previous study has shown that PCMT1 polymorphisms rs4552 and rs4816 of the infant are associated with spina bifida in the Californian population. The fetal PCMT1 rs4816 polymorphism may play only a weak role in neural tube defects (NTDs) formation and that gene-environment interactions might be more significant. The protein repair enzyme is known to protect certain neural cells against Bax-induced apoptosis.
References
Zhao H, et al. (2012) Maternal pcmt1 gene polymorphisms and the risk of neural tube defects in a chinese population of lvliang high-risk area. Gene 505 (2): 340-344.Wang F, et al. (2013) Pcmt1 gene polymorphisms, maternal folate metabolism, and neural tube defects: A case-control study in a population with relatively low folate intake. Genes Nutr 8 (6): 581-587.Wagner AM, et al. (2008) Posttranslational protein modifications in type 1 diabetes-genetic studies with pcmt1, the repair enzyme protein isoaspartate methyltransferase (pimt) encoding gene. Rev Diabet Stud 5 (4): 225-231.Farrar C, et al. (2002) Altered levels of s-adenosylmethionine and s-adenosylhomocysteine in the brains of l-isoaspartyl (d-aspartyl) o-methyltransferase-deficient mice. J Biol Chem 277 (31): 27856-27863.
Similar Products
Product Notes
The PCMT1 (Catalog #AAA256358) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PCMT1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCMT1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PCMT1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCMT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.