WB (Western Blot)
(Detection of mouse PCNA by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody (AAA23771 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human, Mouse PCNA Polyclonal Antibody | anti-PCNA antibody
Rabbit anti-PCNA Antibody, Affinity Purified
IP: 6ug/mg lysate
IHC: 1:2000 to 1:10,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
IHC-IF: 1:1000-1:5000
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse PCNA by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody (AAA23771 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of mouse PCNA by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody (AAA23771 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human PCNA by western blot. Samples: Whole cell lysate (10 ug) from A-549, Jurkat, HEK293T, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody (AAA23771 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human PCNA by western blot. Samples: Whole cell lysate (10 ug) from A-549, Jurkat, HEK293T, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody (AAA23771 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human PCNA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PCNA antibody (AAA23771 lot 2) used for IP at 6 ug per reaction. PCNA was also immunoprecipitated by a previous lot of this antibody (AAA23771 lot 1) and a second antibody against a different epitope of PCNA . For blotting immunoprecipitated PCNA, AAA23771 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human PCNA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PCNA antibody (AAA23771 lot 2) used for IP at 6 ug per reaction. PCNA was also immunoprecipitated by a previous lot of this antibody (AAA23771 lot 1) and a second antibody against a different epitope of PCNA . For blotting immunoprecipitated PCNA, AAA23771 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human PCNA by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-PCNA (Cat. No. AAA23771 Lot1) used at a dilution of 1:4,000 (0.25 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.)
IHC (Immunohistochemistry)
(Detection of human PCNA by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-PCNA (Cat. No. AAA23771 Lot1) used at a dilution of 1:4,000 (0.25 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.)
IHC (Immunohistochemistry)
(Detection of mouse PCNA by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-PCNA (Cat. No. AAA23771 Lot2) used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse PCNA by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-PCNA (Cat. No. AAA23771 Lot2) used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human PCNA by immunohistochemistry. Sample: FFPE section of human breast. Antibody: Affinity purified rabbit anti-PCNA (Cat. No. AAA23771 Lot2) used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human PCNA by immunohistochemistry. Sample: FFPE section of human breast. Antibody: Affinity purified rabbit anti-PCNA (Cat. No. AAA23771 Lot2) used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB)
NCBI and Uniprot Product Information
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Product Notes
The PCNA pcna (Catalog #AAA23771) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PCNA Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PCNA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry-Immunofluorescence (IHC-IF). WB: 1:5000-1:20,000 IP: 6ug/mg lysate IHC: 1:2000 to 1:10,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. IHC-IF: 1:1000-1:5000. Researchers should empirically determine the suitability of the PCNA pcna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCNA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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