WB (Western Blot)
(Western blot analysis of extracts from MCF7, using PCSK9 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 55 kDa.)
Rabbit PCSK9 Polyclonal Antibody | anti-PCSK9 antibody
PCSK9 Antibody
Gene Names
PCSK9; FH3; PC9; FHCL3; NARC1; LDLCQ1; NARC-1; HCHOLA3
Reactivity
Human, Mouse, RatPredicted: Pig, Zebrafish, Horse, Rabbit, Xenopus
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
PCSK9, Antibody; PCSK9 Antibody; Convertase subtilisin/kexin type 9 preproprotein; FH3; HCHOLA3; Hypercholesterolemia autosomal dominant 3; LDLCQ1; NARC 1; NARC-1; NARC1; Neural apoptosis regulated convertase 1; Neural apoptosis-regulated convertase 1; PC 9; PC9; PCSK 9; PCSK9; PCSK9_HUMAN; Proprotein convertase 9; Proprotein convertase PC9; Proprotein convertase subtilisin/kexin type 9; PSEC0052; Subtilisin/kexin like protease PC9; Subtilisin/kexin-like protease PC9; anti-PCSK9 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Horse, Rabbit, Xenopus
Predicted: Pig, Zebrafish, Horse, Rabbit, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
PCSK9 Antibody detects endogenous levels of PCSK9.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
692
Applicable Applications for anti-PCSK9 antibody
WB (Western Blot)
Immunogen
A synthesized peptide derived from human PCSK9.
Tissue Specificity
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Lysosome; Endosome; Endoplasmic Reticulum; Golgi Apparatus; Extracellular Region or Secreted
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from MCF7, using PCSK9 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 55 kDa.)
WB (Western Blot)
(Western blot analysis of extracts from MCF7, using PCSK9 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 55 kDa.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using PCSK9 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using PCSK9 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-PCSK9 antibody
Description: Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments (PubMed:18039658). Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation (PubMed:18799458, PubMed:17461796, PubMed:18197702, PubMed:22074827). Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway (PubMed:18660751). Inhibits epithelial Na+ channel (ENaC)-mediated Na+ absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
Post Translational Modifications: Cleavage by furin and PCSK5 generates a truncated inactive protein that is unable to induce LDLR degradation. Undergoes autocatalytic cleavage in the endoplasmic reticulum to release the propeptide from the N-terminus and the cleavage of the propeptide is strictly required for its maturation and activation. The cleaved propeptide however remains associated with the catalytic domain through non-covalent interactions, preventing potential substrates from accessing its active site. As a result, it is secreted from cells as a propeptide-containing, enzymatically inactive protein. Phosphorylation protects the propeptide against proteolysis.
Subunit Structure: Monomer. Can self-associate to form dimers and higher multimers which may have increased LDLR degrading activity. The precursor protein but not the mature protein may form multimers. Interacts with APOB, VLDLR, LRP8/APOER2 and BACE1. The full-length immature form (pro-PCSK9) interacts with SCNN1A, SCNN1B and SCNN1G. The pro-PCSK9 form (via C-terminal domain) interacts with LDLR. Interacts (via the C-terminal domain) with ANXA2 (via repeat Annexin 1); the interaction inhibits the degradation of LDLR (PubMed:18799458).
Similarity: The C-terminal domain (CRD) is essential for the LDLR-binding and degrading activities. The catalytic domain is responsible for mediating its self-association. Belongs to the peptidase S8 family.
Post Translational Modifications: Cleavage by furin and PCSK5 generates a truncated inactive protein that is unable to induce LDLR degradation. Undergoes autocatalytic cleavage in the endoplasmic reticulum to release the propeptide from the N-terminus and the cleavage of the propeptide is strictly required for its maturation and activation. The cleaved propeptide however remains associated with the catalytic domain through non-covalent interactions, preventing potential substrates from accessing its active site. As a result, it is secreted from cells as a propeptide-containing, enzymatically inactive protein. Phosphorylation protects the propeptide against proteolysis.
Subunit Structure: Monomer. Can self-associate to form dimers and higher multimers which may have increased LDLR degrading activity. The precursor protein but not the mature protein may form multimers. Interacts with APOB, VLDLR, LRP8/APOER2 and BACE1. The full-length immature form (pro-PCSK9) interacts with SCNN1A, SCNN1B and SCNN1G. The pro-PCSK9 form (via C-terminal domain) interacts with LDLR. Interacts (via the C-terminal domain) with ANXA2 (via repeat Annexin 1); the interaction inhibits the degradation of LDLR (PubMed:18799458).
Similarity: The C-terminal domain (CRD) is essential for the LDLR-binding and degrading activities. The catalytic domain is responsible for mediating its self-association. Belongs to the peptidase S8 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 78 kDa
Predicted: 75 kDa
Predicted: 75 kDa
NCBI Official Full Name
proprotein convertase subtilisin/kexin type 9 preproprotein
NCBI Official Synonym Full Names
proprotein convertase subtilisin/kexin type 9
NCBI Official Symbol
PCSK9
NCBI Official Synonym Symbols
FH3; PC9; FHCL3; NARC1; LDLCQ1; NARC-1; HCHOLA3
NCBI Protein Information
proprotein convertase subtilisin/kexin type 9
UniProt Protein Name
Proprotein convertase subtilisin/kexin type 9
UniProt Gene Name
PCSK9
UniProt Synonym Gene Names
NARC1; NARC-1; PC9
UniProt Entry Name
PCSK9_HUMAN
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Product Notes
The PCSK9 pcsk9 (Catalog #AAA328698) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PCSK9 Antibody reacts with Human, Mouse, Rat Predicted: Pig, Zebrafish, Horse, Rabbit, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's PCSK9 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the PCSK9 pcsk9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCSK9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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