IF (Immunofluorescence)
(AAA322586 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human, Mouse PDE10A Polyclonal Antibody | anti-PDE10A antibody
PDE10A Antibody
Gene Names
PDE10A; ADSD2; IOLOD; HSPDE10A; PDE10A19; LINC00473
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PDE10A, Antibody; PDE10A Antibody; 5''-cyclic phosphodiesterase 10A; cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A; cAMP and cAMP-inhibited cGMP 3''; FLJ11894; HSPDE 10A; HSPDE10A; PDE 10A; PDE10_HUMAN; Pde10a; Phosphodiesterase 10A; Phosphodiesterase 10A1; anti-PDE10A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
PDE10A antibody detects endogenous levels of total PDE10A
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
789
Applicable Applications for anti-PDE10A antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human PDE10A
Subcellular Location
Cytoplasm. Located mostly to soluble cellular fractions.
Tissue Specificity
Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.
Predicted Cross Reactivity
Pig, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (91%), Horse (91%), Sheep (91%), Rabbit (91%), Dog (91%), Chicken (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA322586 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA322586 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of PDE10A using HeLa whole cell lysates)
WB (Western Blot)
(Western blot analysis of PDE10A using HeLa whole cell lysates)
Related Product Information for anti-PDE10A antibody
Function: Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. May play a critical role in regulating cAMP and cGMP levels in the striatum, a region of the brain that contributes to the control of movement and cognition.
Subunit Structure: Homodimer.
Post-translational Modifications: Isoform PDE10A2: Phosphorylated on Thr-16.
Similarity: The tandem GAF domains bind cAMP, and regulate enzyme activity. The binding of cAMP stimulates enzyme activity.Composed of a C-terminal catalytic domain containing two divalent metal sites and an N-terminal regulatory domain which contains one cyclic nucleotide-binding region. Belongs to the cyclic nucleotide phosphodiesterase family.
Subunit Structure: Homodimer.
Post-translational Modifications: Isoform PDE10A2: Phosphorylated on Thr-16.
Similarity: The tandem GAF domains bind cAMP, and regulate enzyme activity. The binding of cAMP stimulates enzyme activity.Composed of a C-terminal catalytic domain containing two divalent metal sites and an N-terminal regulatory domain which contains one cyclic nucleotide-binding region. Belongs to the cyclic nucleotide phosphodiesterase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 75 kDa
Predicted: 89 kDa
Predicted: 89 kDa
NCBI Official Full Name
cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A isoform 1
NCBI Official Synonym Full Names
phosphodiesterase 10A
NCBI Official Symbol
PDE10A
NCBI Official Synonym Symbols
ADSD2; IOLOD; HSPDE10A; PDE10A19; LINC00473
NCBI Protein Information
cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
UniProt Protein Name
cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
UniProt Gene Name
PDE10A
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Product Notes
The PDE10A pde10a (Catalog #AAA322586) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDE10A Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PDE10A can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the PDE10A pde10a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE10A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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