<
FCM/FACS (Flow Cytometry)
(PDGFRA Antibody (C-term E1063) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit PDGFRA Polyclonal Antibody | anti-PDGFRA antibody
PDGFRA Antibody (C-term E1063)
Gene Names
PDGFRA; CD140A; PDGFR2; PDGFR-2; RHEPDGFRA
Reactivity
Human, mouse (Predicted Reactivity: Chicken, Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PDGFRA, Antibody; PDGFRA Antibody (C-term E1063); Platelet-derived growth factor receptor alpha; PDGF-R-alpha; PDGFR-alpha; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; PDGFR-2; CD140a; PDGFRA; PDGFR2; RHEPDGFRA; anti-PDGFRA antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Chicken, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1048-1077 amino acids from the C-terminal region of human PDGFRA.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
1048-1077
Sequence Length
743
Applicable Applications for anti-PDGFRA antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(PDGFRA Antibody (C-term E1063) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PDGFRA Antibody (C-term E1063) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Western blot analysis of PDGFRA antibody (C-term E1063) in mouse lung tissue lysates (35ug/lane). PDGFRA (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of PDGFRA antibody (C-term E1063) in mouse lung tissue lysates (35ug/lane). PDGFRA (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of PDGFRA Antibody (C-term E1063) in K562 cell line lysates (35ug/lane). PDGFRA (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of PDGFRA Antibody (C-term E1063) in K562 cell line lysates (35ug/lane). PDGFRA (arrow) was detected using the purified Pab.)
Related Product Information for anti-PDGFRA antibody
PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
Product Categories/Family for anti-PDGFRA antibody
References
Cools, J., et al., N. Engl. J. Med. 348(13):1201-1214 (2003).
Heinrich, M.C., et al., Science 299(5607):708-710 (2003).
Andrae, J., et al., Biochem. Biophys. Res. Commun. 296(3):604-611 (2002).
Ribom, D., et al., J. Neurol. Neurosurg. Psychiatr. 72(6):782-787 (2002).
Kawagishi, J., et al., Genomics 30(2):224-232 (1995).
Heinrich, M.C., et al., Science 299(5607):708-710 (2003).
Andrae, J., et al., Biochem. Biophys. Res. Commun. 296(3):604-611 (2002).
Ribom, D., et al., J. Neurol. Neurosurg. Psychiatr. 72(6):782-787 (2002).
Kawagishi, J., et al., Genomics 30(2):224-232 (1995).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
122670
NCBI Official Full Name
platelet-derived growth factor receptor alpha
NCBI Official Synonym Full Names
platelet-derived growth factor receptor, alpha polypeptide
NCBI Official Symbol
PDGFRA
NCBI Official Synonym Symbols
CD140A; PDGFR2; PDGFR-2; RHEPDGFRA
NCBI Protein Information
platelet-derived growth factor receptor alpha
UniProt Protein Name
Platelet-derived growth factor receptor alpha
UniProt Gene Name
PDGFRA
UniProt Synonym Gene Names
PDGFR2; RHEPDGFRA; PDGF-R-alpha; PDGFR-alpha; PDGFR-2
UniProt Entry Name
PGFRA_HUMAN
Similar Products
Product Notes
The PDGFRA pdgfra (Catalog #AAA285531) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1048-1077. The PDGFRA Antibody (C-term E1063) reacts with Human, mouse (Predicted Reactivity: Chicken, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PDGFRA can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PDGFRA pdgfra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDGFRA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.