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WB (Western Blot)
(Western blot analysis of anti-PDGFRB Antibody (N-term) in cell line lysates (35ug/lane). PDGFRB(arrow) was detected using the purified Pab).)
Rabbit anti-Human PDGFRB Polyclonal Antibody | anti-PDGFRB antibody
PDGFRB Antibody (N-term)
Gene Names
PDGFRB; IMF1; IBGC4; JTK12; PDGFR; CD140B; PDGFR1; PDGFR-1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PDGFRB, Antibody; PDGFRB Antibody (N-term); Platelet-derived growth factor receptor beta; PDGF-R-beta; PDGFR-beta; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1; CD140b; PDGFRB; PDGFR; PDGFR1; anti-PDGFRB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PDGFRB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-72 amino acids from the N-terminal region of human PDGFRB.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
40-72
Sequence Length
336
Applicable Applications for anti-PDGFRB antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Cellular Location
Cell membrane; Single-pass type I membraneprotein. Cytoplasmic vesicle. Lysosomelumen. Note=After ligand binding, theautophosphorylated receptor is ubiquitinatedand internalized, leading to its degradation Provided below are standard protocols that youmay find useful for product applications.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of anti-PDGFRB Antibody (N-term) in cell line lysates (35ug/lane). PDGFRB(arrow) was detected using the purified Pab).)
WB (Western Blot)
(Western blot analysis of anti-PDGFRB Antibody (N-term) in cell line lysates (35ug/lane). PDGFRB(arrow) was detected using the purified Pab).)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of SK-Br-3 cells using PDGFRB Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of SK-Br-3 cells using PDGFRB Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of PDGFRB Antibody (N-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of PDGFRB Antibody (N-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PDGFRB Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PDGFRB Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-PDGFRB antibody
PDGFRB is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses the gene to that of the translocation, ETS, leukemia gene, results in chronic myelomonocytic leukemia.
Tyrosine-protein kinase that acts ascell-surface receptor for homodimeric PDGFBand PDGFD and for heterodimers formed byPDGFA and PDGFB, and plays an essentialrole in the regulation of embryonicdevelopment, cell proliferation, survival,differentiation, chemotaxis and migration.Plays an essential role in blood vesseldevelopment by promoting proliferation,migration and recruitment of pericytes andsmooth muscle cells to endothelial cells.Plays a role in the migration of vascularsmooth muscle cells and the formation ofneointima at vascular injury sites. Requiredfor normal development of the cardiovascularsystem. Required for normal recruitment ofpericytes (mesangial cells) in the kidneyglomerulus, and for normal formation of abranched network of capillaries in kidneyglomeruli. Promotes rearrangement of theactin cytoskeleton and the formation ofmembrane ruffles. Binding of its cognateligands - homodimeric PDGFB, heterodimersformed by PDGFA and PDGFB or homodimericPDGFD -leads to the activation of severalsignaling cascades; the response depends onthe nature of the bound ligand and ismodulated by the formation of heterodimersbetween PDGFRA and PDGFRB.Phosphorylates PLCG1, PIK3R1, PTPN11,RASA1/GAP, CBL, SHC1 and NCK1. Activationof PLCG1 leads to the production of thecellular signaling molecules diacylglyceroland inositol 1,4,5-trisphosphate, mobilizationof cytosolic Ca(2+) and the activation ofprotein kinase C. Phosphorylation of PIK3R1,the regulatory subunit of phosphatidylinositol3-kinase, leads to the activation of the AKT1signaling pathway. Phosphorylation of SHC1,or of the C-terminus of PTPN11, creates abinding site for GRB2, resulting in theactivation of HRAS, RAF1 and down-streamMAP kinases, including MAPK1/ERK2 and/orMAPK3/ERK1. Promotes phosphorylation andactivation of SRC family kinases. Promotesphosphorylation of PDCD6IP/ALIX and STAM.Receptor signaling is down-regulated byprotein phosphatases that dephosphorylatethe receptor and its down-stream effectors,and by rapid internalization of the activatedreceptor
Tyrosine-protein kinase that acts ascell-surface receptor for homodimeric PDGFBand PDGFD and for heterodimers formed byPDGFA and PDGFB, and plays an essentialrole in the regulation of embryonicdevelopment, cell proliferation, survival,differentiation, chemotaxis and migration.Plays an essential role in blood vesseldevelopment by promoting proliferation,migration and recruitment of pericytes andsmooth muscle cells to endothelial cells.Plays a role in the migration of vascularsmooth muscle cells and the formation ofneointima at vascular injury sites. Requiredfor normal development of the cardiovascularsystem. Required for normal recruitment ofpericytes (mesangial cells) in the kidneyglomerulus, and for normal formation of abranched network of capillaries in kidneyglomeruli. Promotes rearrangement of theactin cytoskeleton and the formation ofmembrane ruffles. Binding of its cognateligands - homodimeric PDGFB, heterodimersformed by PDGFA and PDGFB or homodimericPDGFD -leads to the activation of severalsignaling cascades; the response depends onthe nature of the bound ligand and ismodulated by the formation of heterodimersbetween PDGFRA and PDGFRB.Phosphorylates PLCG1, PIK3R1, PTPN11,RASA1/GAP, CBL, SHC1 and NCK1. Activationof PLCG1 leads to the production of thecellular signaling molecules diacylglyceroland inositol 1,4,5-trisphosphate, mobilizationof cytosolic Ca(2+) and the activation ofprotein kinase C. Phosphorylation of PIK3R1,the regulatory subunit of phosphatidylinositol3-kinase, leads to the activation of the AKT1signaling pathway. Phosphorylation of SHC1,or of the C-terminus of PTPN11, creates abinding site for GRB2, resulting in theactivation of HRAS, RAF1 and down-streamMAP kinases, including MAPK1/ERK2 and/orMAPK3/ERK1. Promotes phosphorylation andactivation of SRC family kinases. Promotesphosphorylation of PDCD6IP/ALIX and STAM.Receptor signaling is down-regulated byprotein phosphatases that dephosphorylatethe receptor and its down-stream effectors,and by rapid internalization of the activatedreceptor
Product Categories/Family for anti-PDGFRB antibody
References
Wu, H., et al., J. Biol. Chem. 278(42):40425-40428 (2003).
Whiteman, E.L., et al., Endocrinology 144(9):3811-3820 (2003).
Uren, A., et al., Oncogene 22(15):2334-2342 (2003).
Steer, E.J., et al., Acta Haematol. 107(2):113-122 (2002).
Kawagishi, J., et al., Genomics 30(2):224-232 (1995).
Whiteman, E.L., et al., Endocrinology 144(9):3811-3820 (2003).
Uren, A., et al., Oncogene 22(15):2334-2342 (2003).
Steer, E.J., et al., Acta Haematol. 107(2):113-122 (2002).
Kawagishi, J., et al., Genomics 30(2):224-232 (1995).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
platelet-derived growth factor receptor beta
NCBI Official Synonym Full Names
platelet-derived growth factor receptor, beta polypeptide
NCBI Official Symbol
PDGFRB
NCBI Official Synonym Symbols
IMF1; IBGC4; JTK12; PDGFR; CD140B; PDGFR1; PDGFR-1
NCBI Protein Information
platelet-derived growth factor receptor beta
UniProt Protein Name
Platelet-derived growth factor receptor beta
UniProt Gene Name
PDGFRB
UniProt Synonym Gene Names
PDGFR; PDGFR1; PDGF-R-beta; PDGFR-beta; PDGFR-1
UniProt Entry Name
PGFRB_HUMAN
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Product Notes
The PDGFRB pdgfrb (Catalog #AAA290379) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 40-72. The PDGFRB Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDGFRB can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the PDGFRB pdgfrb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDGFRB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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