IHC (Immunohiostchemistry)
(PDHA2 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PDHA2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human PDHA2 Polyclonal Antibody | anti-PDHA2 antibody
PDHA2 Antibody (Center)
Gene Names
PDHA2; PDHAL
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PDHA2, Antibody; PDHA2 Antibody (Center); Pyruvate dehydrogenase E1 component subunit alpha; testis-specific form; mitochondrial; PDHE1-A type II; PDHA2; PDHAL; anti-PDHA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PDHA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-314 amino acids from the Central region of human PDHA2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
287-314
Sequence Length
388
Applicable Applications for anti-PDHA2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(PDHA2 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PDHA2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(PDHA2 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PDHA2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(PDHA2 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PDHA2 antibody detected the PDHA2 protein (arrow).)
WB (Western Blot)
(PDHA2 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PDHA2 antibody detected the PDHA2 protein (arrow).)
Related Product Information for anti-PDHA2 antibody
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Product Categories/Family for anti-PDHA2 antibody
References
Pinheiro, A., et al. Mol. Genet. Metab. 99(4):425-430(2010)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Caruso, M., et al. J. Biol. Chem. 276(48):45088-45097(2001)
Jacobia, S.J., et al. Arch. Biochem. Biophys. 395(1):121-128(2001)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Caruso, M., et al. J. Biol. Chem. 276(48):45088-45097(2001)
Jacobia, S.J., et al. Arch. Biochem. Biophys. 395(1):121-128(2001)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42933
NCBI Official Full Name
pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
NCBI Official Synonym Full Names
pyruvate dehydrogenase (lipoamide) alpha 2
NCBI Official Symbol
PDHA2
NCBI Official Synonym Symbols
PDHAL
NCBI Protein Information
pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
UniProt Protein Name
Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
UniProt Gene Name
PDHA2
UniProt Synonym Gene Names
PDHAL
UniProt Entry Name
ODPAT_HUMAN
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Product Notes
The PDHA2 pdha2 (Catalog #AAA284288) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 287-314. The PDHA2 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDHA2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PDHA2 pdha2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDHA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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