WB (Western Blot)
(Detection of human and mouse PDIA3 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDIA3 antibody AAA213178 (lot AAA213178-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse PDIA3/ERp57 Polyclonal Antibody | anti-PDIA3/ERp57 antibody
Rabbit anti-PDIA3/ERp57 Antibody, Affinity Purified
Gene Names
PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PDIA3/ERp57, Antibody; Rabbit anti-PDIA3/ERp57 Antibody, Affinity Purified; ERp60; ERp61; glucose regulated protein, 58kDa; GRP57; GRP58; HEL-S-269; HEL-S-93n; P58; PI-PLC; protein disulfide isomerase-associated 3; HsT17083; protein disulfide-isomerase A3; 58 kDa microsomal protein; phospholipase C-alpha; 58 kDa glucose-regulated protein; disulfide isomerase ER-60; endoplasmic reticulum P58; endoplasmic reticulum resident protein 57; epididymis secretory protein Li 269; epididymis secretory sperm binding protein Li 93n; ER protein 57; ER protein 60; ER60; endoplasmic reticulum resident protein 60; ERp57; Protein disulfide-isomerase A3; protein disulfide isomerase family A member 3; anti-PDIA3/ERp57 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PDIA3/ERp57 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Predicted Reactivity
Rat, Bovine, Orangutan
Immunogen
Between 50 and 100
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse PDIA3 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDIA3 antibody AAA213178 (lot AAA213178-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human and mouse PDIA3 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDIA3 antibody AAA213178 (lot AAA213178-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human PDIA3 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-PDIA3 (Cat. No. AAA213178 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human PDIA3 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-PDIA3 (Cat. No. AAA213178 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).)
Related Product Information for anti-PDIA3/ERp57 antibody
Protein disulfide-isomerase A3 (PDIA3) catalyzes the rearrangement of-S-S- bonds in proteins [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P30101].
Product Categories/Family for anti-PDIA3/ERp57 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,782 Da
NCBI Official Full Name
protein disulfide-isomerase A3
NCBI Official Synonym Full Names
protein disulfide isomerase family A, member 3
NCBI Official Symbol
PDIA3
NCBI Official Synonym Symbols
P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083
NCBI Protein Information
protein disulfide-isomerase A3; ER protein 57; ER protein 60; phospholipase C-alpha; 58 kDa microsomal protein; disulfide isomerase ER-60; endoplasmic reticulum P58; 58 kDa glucose-regulated protein; glucose regulated protein, 58kDa; protein disulfide iso
UniProt Protein Name
Protein disulfide-isomerase A3
UniProt Gene Name
PDIA3
UniProt Synonym Gene Names
ERP57; ERP60; GRP58; p58; ER protein 57; ERp57; ER protein 60; ERp60
UniProt Entry Name
PDIA3_HUMAN
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Product Notes
The PDIA3/ERp57 pdia3 (Catalog #AAA213178) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PDIA3/ERp57 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PDIA3/ERp57 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PDIA3/ERp57 pdia3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDIA3/ERp57, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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