IP (Immunoprecipitation)
(PDLIM1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-PDLIM1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PDLIM1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size :36 kDa)
Rabbit anti-Human PDLIM1 Polyclonal Antibody | anti-PDLIM1 antibody
Anti-PDLIM1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PDLIM1, Antibody; Anti-PDLIM1 Antibody, Rabbit Polyclonal; PDLIM1 Antibody, Rabbit PAb, Antigen Affinity Purified; PDZ and LIM domain 1; Anti-CLIM1 Antibody; Anti-CLP-36 Antibody; Anti-CLP36 Antibody; Anti-hCLIM1 Antibody; Anti-HEL-S-112 Antibody; anti-PDLIM1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PDLIM1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PDLIM1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
E Coli-derived Human PDLIM1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PDLIM1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(PDLIM1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-PDLIM1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PDLIM1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size :36 kDa)
IP (Immunoprecipitation)
(PDLIM1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-PDLIM1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PDLIM1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size :36 kDa)
WB (Western Blot)
(Anti-PDLIM1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: 293T Whole Cell LysateLane D: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa)
WB (Western Blot)
(Anti-PDLIM1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: 293T Whole Cell LysateLane D: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa)
Related Product Information for anti-PDLIM1 antibody
PDLIM1 (PDZ And LIM Domain 1) is a Protein Coding gene. The PDLIM1 gene, located on 10q23.33, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. This gene encodes a member of the enigma protein family. The protein contains two protein-interacting domains, a PDZ domain at the amino-terminal end and one to three LIM domains at the carboxyl-terminal. PDLIM1 is widely expressed in the small intestine, colon, and other tissues, and the protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14, and 17. Diseases associated with PDLIM1 include Supravalvular Aortic Stenosis and Donohue Syndrome.
References
Miehe U, et al. (2006) Expression of the actin stress fiber-associated protein clp36 in the human placenta. Histochem Cell Biol 126 (4): 465-471.Liu Z, et al. (2015) Pdz and lim domain protein 1(pdlim1)/clp36 promotes breast cancer cell migration, invasion and metastasis through interaction with alpha-actinin. Oncogene 34 (10): 1300-1311.Kotaka M, et al. (1999) Characterization of the human 36-kda carboxyl terminal lim domain protein (hclim1). J Cell Biochem 72 (2): 279-285.
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Product Notes
The PDLIM1 (Catalog #AAA256464) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PDLIM1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDLIM1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PDLIM1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDLIM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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