IP (Immunoprecipitation)
(Detection of human PDZ-RhoGEF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDZ-RhoGEF antibody AAA211694 used for IP at 3 ug/mg lysate. PDZ-RhoGEF was also immunoprecipitated by rabbit anti-PDZ-RhoGEF antibody which recognizes a downstream epitope. For blotting immunoprecipitated PDZ-RhoGEF, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human PDZ-RhoGEF Polyclonal Antibody | anti-PDZ-RhoGEF antibody
Rabbit anti-PDZ-RhoGEF Antibody, Affinity Purified
Gene Names
ARHGEF11; GTRAP48; PDZ-RHOGEF
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
PDZ-RhoGEF, Antibody; Rabbit anti-PDZ-RhoGEF Antibody, Affinity Purified; rho guanine nucleotide exchange factor 11; RhoA-specific guanine nucleotide exchange factor; Rho guanine nucleotide exchange factor (GEF) 11; Rho guanine exchange factor (GEF) 11; PDZ-RHOGEF; GTRAP48; glutamate transporter EAAT4-associated protein 48; RhoGEF glutamate transport modulator; Rho guanine nucleotide exchange factor 11; anti-PDZ-RhoGEF antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PDZ-RhoGEF antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 150 and 200
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human PDZ-RhoGEF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDZ-RhoGEF antibody AAA211694 used for IP at 3 ug/mg lysate. PDZ-RhoGEF was also immunoprecipitated by rabbit anti-PDZ-RhoGEF antibody which recognizes a downstream epitope. For blotting immunoprecipitated PDZ-RhoGEF, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PDZ-RhoGEF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDZ-RhoGEF antibody AAA211694 used for IP at 3 ug/mg lysate. PDZ-RhoGEF was also immunoprecipitated by rabbit anti-PDZ-RhoGEF antibody which recognizes a downstream epitope. For blotting immunoprecipitated PDZ-RhoGEF, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human PDZ-RhoGEF by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PDZ-RhoGEF (Cat. No. AAA211694 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human PDZ-RhoGEF by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PDZ-RhoGEF (Cat. No. AAA211694 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-PDZ-RhoGEF antibody
PDZ-RhoGEF. (PDZ domain-containing Rho guanine nucleotide exchange factor) catalyzes the exchange of GDP for GTP on the GTPase, Rho, to activate downstream signaling pathways. It contains a PDZ [post synaptic density protein (PSD95), Drosophila dis large tumor suppressor (DIgA), and zonula occludens-1 protein (zo-1)] domain which is an 80-90 amino acid structural domain found in signaling proteins in bacteria, yeast, plants, and animals. PDZ-RhoGEF has been shown to be important to cell migration and the regulation of adhesion movement and trailing edge retraction.
Product Categories/Family for anti-PDZ-RhoGEF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
167,704 Da
NCBI Official Full Name
rho guanine nucleotide exchange factor 11 isoform 1
NCBI Official Synonym Full Names
Rho guanine nucleotide exchange factor (GEF) 11
NCBI Official Symbol
ARHGEF11
NCBI Official Synonym Symbols
GTRAP48; PDZ-RHOGEF
NCBI Protein Information
rho guanine nucleotide exchange factor 11; Rho guanine exchange factor (GEF) 11; RhoGEF glutamate transport modulator; RhoA-specific guanine nucleotide exchange factor; glutamate transporter EAAT4-associated protein 48
UniProt Protein Name
Rho guanine nucleotide exchange factor 11
UniProt Gene Name
ARHGEF11
UniProt Synonym Gene Names
KIAA0380
UniProt Entry Name
ARHGB_HUMAN
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Product Notes
The PDZ-RhoGEF arhgef11 (Catalog #AAA211694) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PDZ-RhoGEF Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDZ-RhoGEF can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the PDZ-RhoGEF arhgef11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDZ-RhoGEF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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