Rabbit PEBP1 Polyclonal Antibody | anti-PEBP1 antibody

PEBP1 Antibody

Cat. Num. AAA168965

• Gene Names: PEBP1; PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1; HEL-210; HEL-S-34
• Reactivity: Human, Mouse, Rat
• Applications: Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antigen affinity purification
• Synonyms: PEBP1, Antibody; PEBP1 Antibody; PEBP1; HCNP; PBP; PEBP; RKIP; anti-PEBP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 187

• Applicable Applications for anti-PEBP1 antibody: IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Recombinant protein of human PEBP1
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-5426 / sc-5422 / sc-5424 / sc-5423 / sc-28837
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cell using PEBP1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemisry)

(Immunohistochemistry of paraffin-embedded rat liver using PEBP1 antibody at dilution of 1:200 (200x lens).)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human thyroid cancer using PEBP1 antibody at dilution of 1:200 (400x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using PEBP1 antibody.)

Related Product Information for anti-PEBP1 antibody

Raf kinase inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family that associates with Raf-1 and the MEK and MAP kinases (1). RKIP has been shown to complex with Raf-1, MEK, and ERK (2). Although MEK and ERK can simultaneously bind RKIP, the association between Raf-1 and RKIP and that of RKIP and MEK are mutually exclusive. Thus, RKIP competitively disrupts the Raf-1-MEK complex and effectively terminates signal transmission from Raf-1 to MAP kinases (2). The inhibitory effect of RKIP on MAP kinase signaling is eliminated by PKC phosphorylation of RKIP at Ser153 (3). PKC phosphorylation on Ser153 also promotes the association of RKIP with GRK2, which prevents GRK2-dependent internalization of GPCR (4). RKIP also interacts with modules of the NF-kappaB pathway, including NF-kappaB-inducing kinase (NIK), TAK1, IKKalpha and IKKbeta (5). These interactions antagonize cytokine-induced activation of the NF-kappaB pathway (5). Restoration of RKIP expression is associated with the inhibition of prostate cancer metastasis, implying that RKIP may be a potential clinical target as a suppressor of tumor metastasis through inhibition of vascular invasion (6).

NCBI and Uniprot Product Information

• NCBI GI #: 1352726
• NCBI GeneID: 5037
• Molecular Weight: 21,057 Da
• NCBI Official Full Name: Phosphatidylethanolamine-binding protein 1
• NCBI Official Synonym Full Names: phosphatidylethanolamine binding protein 1
• NCBI Official Symbol: PEBP1
• NCBI Official Synonym Symbols: PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1; HEL-210; HEL-S-34
• NCBI Protein Information: phosphatidylethanolamine-binding protein 1; Raf kinase inhibitory protein; epididymis luminal protein 210; epididymis secretory protein Li 34; hippocampal cholinergic neurostimulating peptide; neuropolypeptide h3; prostatic binding protein; prostatic-binding protein; raf kinase inhibitor protein
• UniProt Protein Name: Phosphatidylethanolamine-binding protein 1
• UniProt Gene Name: PEBP1
• UniProt Synonym Gene Names: PBP; PEBP; PEBP-1; RKIP; HCNP
• UniProt Entry Name: PEBP1_HUMAN