Application Data
(Anti-Perforin Picoband antibody, AAA46036-2.jpgAll lanes: Anti Perforin (AAA46036) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: COLO320 Whole Cell Lysate at 40ugLane 3: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 61KDObserved bind size: 61KD)
Rabbit Perforin-1 Polyclonal Antibody | anti-PRF1 antibody
Anti-Perforin Antibody
Gene Names
PRF1; P1; PFP; FLH2; PFN1; HPLH2
Reactivity
Human. No cross reactivity with other proteins.
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
Perforin-1, Antibody; Anti-Perforin Antibody; Cytolysin antibody; FLH2 antibody; HPLH2 antibody; Lymphocyte pore forming protein antibody; Lymphocyte pore-forming protein antibody; MGC65093 antibody; P1 antibody; PERF_HUMAN antibody; perforin 1 (pore forming protein) antibody; Perforin 1 antibody; Perforin 1 precursor antibody; Perforin 1 preforming protein antibody; Perforin-1 antibody; PFP antibody; PGFL antibody; PIGF antibody; PIGF-2 antibody; PLGF antibody; Pore forming protein antibody; PRF 1 antibody; PRF1 antibody; SHGC-10760 antibody; perforin 1 (pore forming protein); anti-PRF1 antibody
Host
Rabbit
Reactivity
Human. No cross reactivity with other proteins.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
555
Applicable Applications for anti-PRF1 antibody
WB (Western Blot)
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E.coli-derived human Perforin recombinant protein (Position: E175-W555). Human Perforin shares 68% amino acid (aa) sequence identity with both mouse and rat Perforin.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Application Data
(Anti-Perforin Picoband antibody, AAA46036-2.jpgAll lanes: Anti Perforin (AAA46036) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: COLO320 Whole Cell Lysate at 40ugLane 3: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 61KDObserved bind size: 61KD)
Application Data
(Anti-Perforin Picoband antibody, AAA46036-2.jpgAll lanes: Anti Perforin (AAA46036) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: COLO320 Whole Cell Lysate at 40ugLane 3: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 61KDObserved bind size: 61KD)
Application Data
(Anti-Perforin Picoband antibody, AAA46036-1.jpgAll lanes: Anti Perforin (AAA46036) at 0.5ug/mlWB: Recombinant Human Perforin Protein 0.5ngPredicted bind size: 40KDObserved bind size: 40KD)
Application Data
(Anti-Perforin Picoband antibody, AAA46036-1.jpgAll lanes: Anti Perforin (AAA46036) at 0.5ug/mlWB: Recombinant Human Perforin Protein 0.5ngPredicted bind size: 40KDObserved bind size: 40KD)
Related Product Information for anti-PRF1 antibody
Description: Rabbit IgG polyclonal antibody for Perforin-1(PRF1) detection. Tested with WB in Human.
Background: PRF1, also known as Perforin-1, is a protein that in humans is encoded by the PRF1 gene. It is mapped to 10q22.1. PRF1 is a cytolytic protein found in the granules of Cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, PRF1 inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria. PRF1 has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. PRF1 is thought to act by creating holes in the plasma membrane which triggers an influx of calcium and initiates membrane repair mechanisms. These repair mechanisms bring perforin and granzymes into early endosomes.
Background: PRF1, also known as Perforin-1, is a protein that in humans is encoded by the PRF1 gene. It is mapped to 10q22.1. PRF1 is a cytolytic protein found in the granules of Cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, PRF1 inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria. PRF1 has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. PRF1 is thought to act by creating holes in the plasma membrane which triggers an influx of calcium and initiates membrane repair mechanisms. These repair mechanisms bring perforin and granzymes into early endosomes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,377 Da
NCBI Official Full Name
perforin-1
NCBI Official Synonym Full Names
perforin 1 (pore forming protein)
NCBI Official Symbol
PRF1
NCBI Official Synonym Symbols
P1; PFP; FLH2; PFN1; HPLH2
NCBI Protein Information
perforin-1; cytolysin; lymphocyte pore forming protein; lymphocyte pore-forming protein
UniProt Protein Name
Perforin-1
UniProt Gene Name
PRF1
UniProt Synonym Gene Names
PFP; P1; PFP
UniProt Entry Name
PERF_HUMAN
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Product Notes
The PRF1 prf1 (Catalog #AAA46036) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Perforin Antibody reacts with Human. No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Perforin-1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the PRF1 prf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Perforin-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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