IF (Immunofluorescence)
(Immunofluorescence staining of PCNT in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PCNT polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Centrosome.)
Rabbit Pericentrin Polyclonal Antibody | anti-PCNT antibody
Anti-Pericentrin Antibody, Rabbit Polyclonal
Applications
Immunofluorescence, Immunocytochemistry
Purity
Antigen Affinity Purified
Synonyms
Pericentrin, Antibody; Anti-Pericentrin Antibody, Rabbit Polyclonal; Pericentrin/PCNT Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-KIAA0402 Antibody; Anti-PCNT2 Antibody; anti-PCNT antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Pericentrin/PCNT
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PCNT antibody
IF (Immunofluorescence), ICC (Immunocytochemistry)
Immunogen
E Coli-derived Human Pericentrin/PCNT fragment
Preparation
Produced in rabbits immunized with E Coli-derived Human Pericentrin/PCNT fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of PCNT in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PCNT polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Centrosome.)
IF (Immunofluorescence)
(Immunofluorescence staining of PCNT in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PCNT polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Centrosome.)
References
Sepulveda G, et al. (2018) Co-translational protein targeting facilitates centrosomal recruitment of pcnt during centrosome maturation in vertebrates. Elife 7: e34959.Lorenzo-Betancor O, et al. (2018) Pcnt point mutations and familial intracranial aneurysms. Neurology 91 (23): e2170-e2181.
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Product Notes
The PCNT (Catalog #AAA256312) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Pericentrin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the PCNT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pericentrin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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