IHC (Immunohiostchemistry)
(Staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit Perilipin-1 Polyclonal Antibody | anti-PLIN1 antibody
Phospho-Perilipin-1 (Ser522) Antibody
Gene Names
PLIN1; PERI; PLIN; FPLD4
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
Perilipin-1, Antibody; Phospho-Perilipin-1 (Ser522) Antibody; Lipid droplet associated protein; Lipid droplet-associated protein; PERI; Perilipin A; Perilipin; Perilipin-1; PerilipinA; PLIN; PLIN1; PLIN1_HUMAN; anti-PLIN1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-Perilipin-1 (Ser522) Antibody detects endogenous levels of Perilipin-1 only when phosphorylated at Ser522.
Tissue Specificity: Detected in adipocytes from white adipose tissue (at protein level). Detected in visceral adipose tissue and mammary gland.
Tissue Specificity: Detected in adipocytes from white adipose tissue (at protein level). Detected in visceral adipose tissue and mammary gland.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PLIN1 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry)
Immunogen
A synthesized peptide derived from human Perilipin-1 around the phosphorylation site of Ser522.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet (By similarity).
Subunit Structure
Interacts with ABHD5 (By similarity). Interacts with CIDEC. Interacts with AQP7.
Similarity
Belongs to the perilipin family.
Subcellular Location
Endoplasmic reticulum. Lipid droplet.
Note: Lipid droplet surface-associated.
Note: Lipid droplet surface-associated.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(Staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(At 1/100 staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(At 1/100 staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Related Product Information for anti-PLIN1 antibody
Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,990 Da
NCBI Official Full Name
perilipin-1
NCBI Official Synonym Full Names
perilipin 1
NCBI Official Symbol
PLIN1
NCBI Official Synonym Symbols
PERI; PLIN; FPLD4
NCBI Protein Information
perilipin-1; lipid droplet-associated protein
UniProt Protein Name
Perilipin-1
UniProt Gene Name
PLIN1
UniProt Synonym Gene Names
PERI; PLIN
UniProt Entry Name
PLIN1_HUMAN
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Product Notes
The PLIN1 plin1 (Catalog #AAA329331) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Perilipin-1 (Ser522) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Perilipin-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the PLIN1 plin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Perilipin-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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