FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U251 cells using anti-Peroxiredoxin 2/PRDX2 antibody (AAA127090).Overlay histogram showing U251 cells stained with AAA127090 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Peroxiredoxin 2/PRDX2 Antibody (AAA127090, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit anti-Human peroxiredoxin 2/PRDX2 Polyclonal Antibody | anti-PRDX2 antibody
Anti-peroxiredoxin 2/PRDX2 Antibody Picoband
Gene Names
PRDX2; PRP; TSA; PRX2; PTX1; TPX1; NKEFB; PRXII; TDPX1; NKEF-B
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
peroxiredoxin 2/PRDX2, Antibody; Anti-peroxiredoxin 2/PRDX2 Antibody Picoband; cholinergic receptor nicotinic alpha 3 subunit ; Neuronal acetylcholine receptor subunit alpha-3; CHRNA3; NACHRA3; anti-PRDX2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-PRDX2 antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E coli-derived human peroxiredoxin 2/PRDX2 recombinant protein (Position: N5-R127).
Subcellular Localization
Cell junction, synapse, postsynaptic cell membrane, Multi-pass membrane protein, Cell membrane, Multi-pass membrane protein
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U251 cells using anti-Peroxiredoxin 2/PRDX2 antibody (AAA127090).Overlay histogram showing U251 cells stained with AAA127090 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Peroxiredoxin 2/PRDX2 Antibody (AAA127090, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U251 cells using anti-Peroxiredoxin 2/PRDX2 antibody (AAA127090).Overlay histogram showing U251 cells stained with AAA127090 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Peroxiredoxin 2/PRDX2 Antibody (AAA127090, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of Peroxiredoxin 2/PRDX2 using anti-Peroxiredoxin 2/PRDX2 antibody (AAA127090).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human MCF-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Peroxiredoxin 2/PRDX2 antigen affinity purified polyclonal antibody (#AAA127090) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Peroxiredoxin 2/PRDX2 at approximately 22 kDa. The expected band size for Peroxiredoxin 2/PRDX2 is at 22 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of Peroxiredoxin 2/PRDX2 using anti-Peroxiredoxin 2/PRDX2 antibody (AAA127090).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human MCF-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Peroxiredoxin 2/PRDX2 antigen affinity purified polyclonal antibody (#AAA127090) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Peroxiredoxin 2/PRDX2 at approximately 22 kDa. The expected band size for Peroxiredoxin 2/PRDX2 is at 22 kDa.)
Related Product Information for anti-PRDX2 antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-PRDX2 antibody
References
1. Choi, M. H., Lee, I. K., Kim, G. W., Kim, B. U., Han, Y.-H., Yu, D.-Y., Park, H. S., Kim, K. Y., Lee, J. S., Choi, C., Bae, Y. S., Lee, B. I., Rhee, S. G., Kang, S. W. Regulation of PDGF signalling and vascular remodelling by peroxiredoxin II. Nature 435: 347-353, 2005.2. Lee, T.-H., Kim, S.-U., Yu, S.-L., Kim, S. H., Park, D. S., Moon, H.-B., Dho, S. H., Kwon, K.-S., Kwon, H. J., Han, Y.-H., Jeong, S., Kang, S. W., Shin, H.-S., Lee, K.-K., Rhee, S. G., Yu, D.-Y. Peroxiredoxin II is essential for sustaining life span of erythrocytes in mice. Blood 101: 5033-5038, 2003. 3. Pahl, P., Berger, R., Hart, I., Chae, H. Z., Rhee, S. G., Patterson, D. Localization of TDPX1, a human homologue of the yeast thioredoxin-dependent peroxide reductase gene (TPX), to chromosome 13q12. Genomics 26: 602-606, 1995.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,892 Da
NCBI Official Full Name
PRDX2
NCBI Official Synonym Full Names
peroxiredoxin 2
NCBI Official Symbol
PRDX2
NCBI Official Synonym Symbols
PRP; TSA; PRX2; PTX1; TPX1; NKEFB; PRXII; TDPX1; NKEF-B
NCBI Protein Information
peroxiredoxin-2; torin; thioredoxin peroxidase 1; thiol-specific antioxidant 1; natural killer cell-enhancing factor B; thioredoxin-dependent peroxide reductase 1
UniProt Protein Name
Peroxiredoxin-2
UniProt Gene Name
PRDX2
UniProt Synonym Gene Names
NKEFB; TDPX1; NKEF-B; TSA
UniProt Entry Name
PRDX2_HUMAN
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Product Notes
The PRDX2 prdx2 (Catalog #AAA127090) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-peroxiredoxin 2/PRDX2 Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's peroxiredoxin 2/PRDX2 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the PRDX2 prdx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "peroxiredoxin 2/PRDX2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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