WB (Western Blot)
(Anti-PRDX6 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: MCF7 Whole Cell LysateLane D: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:25 kDa)
Rabbit anti-Human Peroxiredoxin 6 Polyclonal Antibody | anti-PRDX6 antibody
Anti-Peroxiredoxin 6 Antibody, Rabbit Polyclonal
Gene Names
PRDX6; PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; HEL-S-128m
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Peroxiredoxin 6, Antibody; Anti-Peroxiredoxin 6 Antibody, Rabbit Polyclonal; Peroxiredoxin 6/PRDX6 Antibody, Rabbit PAb, Antigen Affinity Purified; peroxiredoxin 6; Anti-1-Cys Antibody; Anti-aiPLA2 Antibody; Anti-AOP2 Antibody; Anti-HEL-S-128m Antibody; Anti-NSGPx Antibody; Anti-p29 Antibody; Anti-PRX Antibody; anti-PRDX6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Peroxiredoxin 6/PRDX6
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PRDX6 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Recombinant Human Peroxiredoxin 6/PRDX6 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human Peroxiredoxin 6/PRDX6 (rh Peroxiredoxin 6/PRDX6). Peroxiredoxin 6/PRDX6 specific IgG was purified by Human Peroxiredoxin 6/PRDX6 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-PRDX6 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: MCF7 Whole Cell LysateLane D: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:25 kDa)
WB (Western Blot)
(Anti-PRDX6 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: MCF7 Whole Cell LysateLane D: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:25 kDa)
IP (Immunoprecipitation)
(PRDX6 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-PRDX6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PRDX6 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size: 25 kDa)
IP (Immunoprecipitation)
(PRDX6 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-PRDX6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PRDX6 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size: 25 kDa)
Related Product Information for anti-PRDX6 antibody
PRDX6, a member of antioxidant protein superfamily, plays an important role in oxidative stress, catabolism of lipids and phospholipid lipisomes. Peroxiredoxin 6 (PRDX6) is involved in redox regulation of the cell and is thought to be protective against oxidant injury. Peroxiredoxin 6 (PRDX6) is a bifunctional protein with both glutathione peroxidase (GPx) and iPLA2 activities, which are concomitantly increased with the expression of PRDX6. PRDX6 promoted lung tumor growth in an in vivo allograft model.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Observed: 27kDa
Calculated: 25kDa
Calculated: 25kDa
NCBI Official Full Name
Peroxiredoxin-6
NCBI Official Synonym Full Names
peroxiredoxin 6
NCBI Official Symbol
PRDX6
NCBI Official Synonym Symbols
PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; HEL-S-128m
NCBI Protein Information
peroxiredoxin-6; 1-Cys PRX; 24 kDa protein; 1-Cys peroxiredoxin; antioxidant protein 2; liver 2D page spot 40; red blood cells page spot 12; non-selenium glutathione peroxidase; acidic calcium-independent phospholipase A2; epididymis secretory sperm bindi
UniProt Protein Name
Peroxiredoxin-6
UniProt Gene Name
PRDX6
UniProt Synonym Gene Names
AOP2; KIAA0106; 1-Cys PRX; aiPLA2; NSGPx
UniProt Entry Name
PRDX6_HUMAN
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Product Notes
The PRDX6 prdx6 (Catalog #AAA255375) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Peroxiredoxin 6 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Peroxiredoxin 6 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PRDX6 prdx6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Peroxiredoxin 6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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