IP (Immunoprecipitation)
(PFDN2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-PFDN2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PFDN2 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa)
Rabbit anti-Human PFDN2 Polyclonal Antibody | anti-PFDN2 antibody
Anti-PFDN2 Antibody, Rabbit Polyclonal
Gene Names
PFDN2; PFD2
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PFDN2, Antibody; Anti-PFDN2 Antibody, Rabbit Polyclonal; PFD2/PFDN2 Antibody, Rabbit PAb, Antigen Affinity Purified; prefoldin subunit 2; Anti-PFD2 Antibody; anti-PFDN2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PFD2/PFDN2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PFDN2 antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Human PFD2/PFDN2 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human PFD2/PFDN2 (rh PFD2/PFDN2). PFD2/PFDN2 specific IgG was purified by Human PFD2/PFDN2 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(PFDN2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-PFDN2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PFDN2 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa)
IP (Immunoprecipitation)
(PFDN2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-PFDN2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PFDN2 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa)
WB (Western Blot)
(Anti-PFDN2 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:17 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-PFDN2 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:17 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-PFDN2 antibody
PFD2, also known as PFDN2, belongs to the prefoldin beta subunit family. PFD2 is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. PFD2 binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. PFD2 also binds to a nascent polypeptide chain and promotes folding in a setting in which there are many competing pathways for nonnative proteins.
References
Gerhard DS, et al. (2004) The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC). Genome Res. 14(10B):2121-7. Bauch A, et al. (2004) A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. Nat Cell Biol. 6(2):97-105. Nogaj LA, et al. (2005) Cellular levels of glutamyl-tRNA reductase and glutamate-1-semialdehyde aminotransferase do not control chlorophyll synthesis in Chlamydomonas reinhardtii. Plant Physiol. 139(1):389-96.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,648 Da
NCBI Official Full Name
prefoldin subunit 2
NCBI Official Synonym Full Names
prefoldin subunit 2
NCBI Official Symbol
PFDN2
NCBI Official Synonym Symbols
PFD2
NCBI Protein Information
prefoldin subunit 2; prefoldin 2
UniProt Protein Name
Prefoldin subunit 2
UniProt Gene Name
PFDN2
UniProt Synonym Gene Names
PFD2
UniProt Entry Name
PFD2_HUMAN
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Product Notes
The PFDN2 pfdn2 (Catalog #AAA255759) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PFDN2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PFDN2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PFDN2 pfdn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFDN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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