Rabbit anti-Human, Rat PFKFB3 Polyclonal Antibody | anti-PFKFB3 antibody

Phospho-PFKFB3 (Ser461) Antibody

Cat. Num. AAA31288

• Gene Names: PFKFB3; PFK2; IPFK2
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: PFKFB3, Antibody; Phospho-PFKFB3 (Ser461) Antibody; 6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3; 6-bisphosphatase; 6-P2ase 3; 6-P2ASE brain/placenta-type isozyme; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2,6 P2ase 3; 6PF-2-K/Fru-2; F263_HUMAN; fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase; Fructose-2; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase; iPFK 2; iPFK-2; IPFK2; PFK/FBPase 3; PFK2; PFKFB3; Renal carcinoma antigen NY REN 56; Renal carcinoma antigen NY-REN-56; uPFK; anti-PFKFB3 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-PFKFB3 (Ser461) Antibody detects endogenous levels of PFKFB3 only when phosphorylated at Ser461.; Tissue Specificity: Ubiquitous.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PFKFB3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
• Application Notes: IHC: 1:50-1:200; IF/ICC: 1:100-1:500; WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human PFKFB3 around the phosphorylation site of Ser461.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylation by AMPK stimulates activity.
• Similarity: In the C-terminal section; belongs to the phosphoglycerate mutase family.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Application Data

(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

IHC (Immunohistchemistry)

(At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/100 staining Rat muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(Staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of extracts from various samples, using Phospho-PFKFB3 (Ser461) Antibody.Lane 1: Rat lung, blocked with antigen-specific peptides,Lane 2: Rat lung,Lane 3: Raji cells.Observed bands: 55kDa)

Related Product Information for anti-PFKFB3 antibody

Synthesis and degradation of fructose 2,6-bisphosphate.

NCBI and Uniprot Product Information

• NCBI GI #: 224282149
• NCBI GeneID: 5209
• NCBI Accession #: NP_001138915.1
• NCBI GenBank Nucleotide #: NM_001145443.1
• UniProt Accession #: Q16875; O43622; O75902; Q5VX19
• Molecular Weight: 59,609 Da
• NCBI Official Full Name: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 2
• NCBI Official Synonym Full Names: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
• NCBI Official Symbol: PFKFB3
• NCBI Official Synonym Symbols: PFK2; IPFK2
• NCBI Protein Information: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; iPFK-2; PFK/FBPase 3; 6PF-2-K/Fru-2,6-P2ase 3; renal carcinoma antigen NY-REN-56; 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase; 6-phosphofr
• UniProt Protein Name: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
• UniProt Gene Name: PFKFB3
• UniProt Synonym Gene Names: 6PF-2-K/Fru-2,6-P2ase 3
• UniProt Entry Name: F263_HUMAN