IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit anti-Human PFKP Polyclonal Antibody | anti-PFKP antibody
PFKP Antibody (C-term)
Gene Names
PFKP; PFKF; PFK-C; PFK-P; ATP-PFK
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PFKP, Antibody; PFKP Antibody (C-term); ATP-dependent 6-phosphofructokinase; platelet type {ECO:0000255|HAMAP-Rule:MF_03184}; ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184}; PFK-P; 27111 {ECO:0000255|HAMAP-Rule:MF_03184}; 6-phosphofructokinase type C; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphohexokinase {ECO:0000255|HAMAP-Rule:MF_03184}; PFKP; PFKF; anti-PFKP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PFKP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 754-784 amino acids from the C-terminal region of human PFKP.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
754-784
Sequence Length
776
Applicable Applications for anti-PFKP antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Fibroblasts were cultured for 2 d in DMEM supplemented with 10% TH-depleted bovine serum. They were then treated with 2 nM T3 in the absence or presence of either LY294002 (LY, 50 M) or PD98059 (PD, 50 M), added 1 h before the addition of T3. Cells were harvested 24 h after T3 treatment. Whole-cell lysates, obtained from two separately treated culture dishes for each treatment, were subjected to WB with anti-PFKP. (Mol. Endocrinol. 2005 Dec 01;19(12):2955-2963))
WB (Western Blot)
(Fibroblasts were cultured for 2 d in DMEM supplemented with 10% TH-depleted bovine serum. They were then treated with 2 nM T3 in the absence or presence of either LY294002 (LY, 50 M) or PD98059 (PD, 50 M), added 1 h before the addition of T3. Cells were harvested 24 h after T3 treatment. Whole-cell lysates, obtained from two separately treated culture dishes for each treatment, were subjected to WB with anti-PFKP. (Mol. Endocrinol. 2005 Dec 01;19(12):2955-2963))
WB (Western Blot)
(PFKP Antibody (D769) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the PFKP antibody detected the PFKP protein (arrow).)
WB (Western Blot)
(PFKP Antibody (D769) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the PFKP antibody detected the PFKP protein (arrow).)
Related Product Information for anti-PFKP antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).
Product Categories/Family for anti-PFKP antibody
References
Adam, G.C., et al., Mol. Cell Proteomics 1(10):828-835 (2002).
Bonaldo, M.F., et al., Genome Res. 6(9):791-806 (1996).
Morrison, N., et al., Hum. Genet. 89(1):105-106 (1992).
Simpson, C.J., et al., Biochem. Biophys. Res. Commun. 180(1):197-203 (1991).
Nakajima, H., et al., FEBS Lett. 223(1):113-116 (1987).
Bonaldo, M.F., et al., Genome Res. 6(9):791-806 (1996).
Morrison, N., et al., Hum. Genet. 89(1):105-106 (1992).
Simpson, C.J., et al., Biochem. Biophys. Res. Commun. 180(1):197-203 (1991).
Nakajima, H., et al., FEBS Lett. 223(1):113-116 (1987).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85596
NCBI Official Full Name
ATP-dependent 6-phosphofructokinase, platelet type isoform 2
NCBI Official Synonym Full Names
phosphofructokinase, platelet
NCBI Official Symbol
PFKP
NCBI Official Synonym Symbols
PFKF; PFK-C; PFK-P; ATP-PFK
NCBI Protein Information
ATP-dependent 6-phosphofructokinase, platelet type
UniProt Protein Name
ATP-dependent 6-phosphofructokinase, platelet type
UniProt Gene Name
PFKP
UniProt Synonym Gene Names
PFKF; ATP-PFK; PFK-P
UniProt Entry Name
PFKAP_HUMAN
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Product Notes
The PFKP pfkp (Catalog #AAA284444) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 754-784. The PFKP Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PFKP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PFKP pfkp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFKP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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