IP (Immunoprecipitation)
(PFN1 was immunoprecipitated using:Lane A:0.5 mg PC12 Whole Cell Lysate4 uL anti-PFN1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PFN1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size :13 kDa)
Rabbit PFN1/Profilin 1 Polyclonal Antibody | anti-PFN1 antibody
Anti-PFN1/Profilin 1 Antibody, Rabbit Polyclonal
Reactivity
Mouse, Rat, Bovin(Species predicted to react based on 100% sequence homology)
Purity
Protein A & Antigen Affinity
Synonyms
PFN1/Profilin 1, Antibody; Anti-PFN1/Profilin 1 Antibody, Rabbit Polyclonal; profilin 1; Anti-Pfn Antibody; anti-PFN1 antibody
Host
Rabbit
Reactivity
Mouse, Rat, Bovin
(Species predicted to react based on 100% sequence homology)
(Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Immunogen
A synthetic peptide corresponding to the center region of the Mouse PFN1/Profilin 1
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse PFN1/Profilin 1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(PFN1 was immunoprecipitated using:Lane A:0.5 mg PC12 Whole Cell Lysate4 uL anti-PFN1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PFN1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size :13 kDa)
IP (Immunoprecipitation)
(PFN1 was immunoprecipitated using:Lane A:0.5 mg PC12 Whole Cell Lysate4 uL anti-PFN1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PFN1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size :13 kDa)
WB (Western Blot)
(Anti-PFN1 rabbit polyclonal antibody at 1:500 dilutionLane A: PC12 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa)
WB (Western Blot)
(Anti-PFN1 rabbit polyclonal antibody at 1:500 dilutionLane A: PC12 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa)
Related Product Information for anti-PFN1 antibody
Mutations in the profilin 1 (PFN1) gene cause amyotrophic lateral sclerosis (ALS), a neurodegenerative disease caused by the loss of motor neurons leading to paralysis and eventually death. PFN1 is a small actin-binding protein that promotes formin-based actin polymerization and regulates numerous cellular functions.
Similar Products
Product Notes
The PFN1 (Catalog #AAA258839) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PFN1/Profilin 1 Antibody, Rabbit Polyclonal reacts with Mouse, Rat, Bovin (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "PFN1/Profilin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.