FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AP22207a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA290847, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight®488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Rabbit PGAP1 Polyclonal Antibody | anti-PGAP1 antibody
PGAP1 Antibody (N-Term)
Gene Names
PGAP1; Bst1; MRT42; SPG67; ISPD3024
Reactivity
Human, Mouse, RatPredicted: Mouse, Rat
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
PGAP1, Antibody; PGAP1 Antibody (N-Term); GPI inositol-deacylase; 3.1.-.-; Post-GPI attachment to proteins factor 1; hPGAP1; PGAP1; anti-PGAP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Clonality
Polyclonal
Isotype
Ig
Specificity
This PGAP1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 90-122 amino acids from human PGAP1.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
748
Applicable Applications for anti-PGAP1 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Cellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AP22207a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA290847, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight®488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AP22207a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA290847, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight®488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PGAP1 with AAA290847 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PGAP1 with AAA290847 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).)
WB (Western Blot)
(All lanes : Anti-PGAP1 Antibody (N-Term) at 1:2000 dilutionLane 1: SH-SY5Y whole cell lysateLane 2: Hela whole cell lysateLane 3: 293 whole cell lysateLane 4: mouse kidney lysateLane 5: mouse testis lysateLane 6: rat kidney lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 105 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-PGAP1 Antibody (N-Term) at 1:2000 dilutionLane 1: SH-SY5Y whole cell lysateLane 2: Hela whole cell lysateLane 3: 293 whole cell lysateLane 4: mouse kidney lysateLane 5: mouse testis lysateLane 6: rat kidney lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 105 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-PGAP1 antibody
Involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi (By similarity).
Product Categories/Family for anti-PGAP1 antibody
References
Tanaka S.,et al.J. Biol. Chem. 279:14256-14263 (2004). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Bechtel S.,et al.BMC Genomics 8:399-399 (2007). Hillier L.W.,et al.Nature 434:724-731 (2005). Clark H.F.,et al.Genome Res. 13:2265-2270 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 105383
NCBI Official Full Name
GPI inositol-deacylase isoform 2
NCBI Official Synonym Full Names
post-GPI attachment to proteins 1
NCBI Official Symbol
PGAP1
NCBI Official Synonym Symbols
Bst1; MRT42; SPG67; ISPD3024
NCBI Protein Information
GPI inositol-deacylase
UniProt Protein Name
GPI inositol-deacylase
UniProt Gene Name
PGAP1
UniProt Synonym Gene Names
hPGAP1
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Product Notes
The PGAP1 pgap1 (Catalog #AAA290847) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PGAP1 Antibody (N-Term) reacts with Human, Mouse, Rat Predicted: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PGAP1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PGAP1 pgap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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