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IF (Immunofluorescence)
(Immunofluorescent analysis of PGF in HepG2 cell lysate. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with PGF polyclonal Ab using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.)
Rabbit PGF Polyclonal Antibody | anti-PGF antibody
PGF Antibody
Gene Names
PGF; PGFL; PIGF; PLGF; PlGF-2; D12S1900; SHGC-10760
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
Synonyms
PGF, Antibody; PGF Antibody; PGF; PGFL; Placenta growth factor; PlGF; anti-PGF antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
Form/Format
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-PGF antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human PGF, corresponding to a region within the internal amino acids.
Important
For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Preparation and Storage
Store at -20 °C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(Immunofluorescent analysis of PGF in HepG2 cell lysate. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with PGF polyclonal Ab using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.)
IF (Immunofluorescence)
(Immunofluorescent analysis of PGF in HepG2 cell lysate. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with PGF polyclonal Ab using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.)
IHC (Immunohistochemisry)
(Immunohistochemistry analysis of paraffin-embedded PGF in mouse colon tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with PGF polyclonal Ab using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.)
IHC (Immunohistochemisry)
(Immunohistochemistry analysis of paraffin-embedded PGF in mouse colon tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with PGF polyclonal Ab using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.)
WB (Western Blot)
(Western blot analysis of extracts from rat muscle, using PGF Ab.)
WB (Western Blot)
(Western blot analysis of extracts from rat muscle, using PGF Ab.)
WB (Western Blot)
(Western blot analysis of Hepg2 whole cell lysates, using PGF Antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of Hepg2 whole cell lysates, using PGF Antibody. The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-PGF antibody
This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.
References
Chen W, Chen J, Xu M, Zhong Z, Zhang Q, Yang W, Huang G; Journal: Biol Reprod. Electroacupuncture facilitates implantation by enhancing endometrial angiogenesis in a rat model of ovarian hyperstimulation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24kDa
NCBI Official Full Name
placenta growth factor isoform 2
NCBI Official Synonym Full Names
placental growth factor
NCBI Official Symbol
PGF
NCBI Official Synonym Symbols
PGFL; PIGF; PLGF; PlGF-2; D12S1900; SHGC-10760
NCBI Protein Information
placenta growth factor
UniProt Protein Name
Placenta growth factor
UniProt Gene Name
PGF
UniProt Synonym Gene Names
PGFL; PLGF; PlGF
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Product Notes
The PGF pgf (Catalog #AAA326345) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PGF Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PGF can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PGF pgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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