IP (Immunoprecipitation)
(H3A was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate1 uL anti-H3A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-H3A rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size :15 kDa)
Rabbit PGM2/phosphoglucomutase 2 Polyclonal Antibody | anti-PGM2 antibody
Anti-PGM2/phosphoglucomutase 2 Antibody, Rabbit Polyclonal
Gene Names
PGM2; MSTP006
Reactivity
HumanPredicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PGM2/phosphoglucomutase 2, Antibody; Anti-PGM2/phosphoglucomutase 2 Antibody, Rabbit Polyclonal; PGM2/phosphoglucomutase 2 Antibody, Rabbit PAb, Antigen Affinity Purified; phosphoglucomutase 2; Anti-MSTP006 Antibody; anti-PGM2 antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PGM2/phosphoglucomutase 2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PGM2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the center region of the Human PGM2/phosphoglucomutase 2
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PGM2/phosphoglucomutase 2, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(H3A was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate1 uL anti-H3A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-H3A rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size :15 kDa)
IP (Immunoprecipitation)
(H3A was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate1 uL anti-H3A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-H3A rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size :15 kDa)
WB (Western Blot)
(Anti-PGM2 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:68 kDaObserved band size:68 kDa)
WB (Western Blot)
(Anti-PGM2 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:68 kDaObserved band size:68 kDa)
Related Product Information for anti-PGM2 antibody
The gene PGM2 encodes the last enzyme of the Leloir pathway, phosphoglucomutase 2 (Pgm2p), which catalyzes the reversible conversion of glucose 1-phosphate to glucose 6-phosphate. Overexpression of PGM2 has previously been shown to enhance the aerobic growth of S. cerevisiae in galactose medium. Overexpression of the PGM2 gene encoding phosphoglucomutase (Pgm2p) has been shown to improve galactose utilization both under aerobic and under anaerobic conditions. PGM2 overexpression in S. cerevisiae from an integrative plasmid is sufficient to reduce the lag phase and to enhance the growth rate in anaerobic galactose fermentation, which results in an overall decrease in fermentation duration. Deletion of PGM2 decreased cellular glucose-1-phosphate and overexpression of SNR84 increased the maltose permease activity.
References
Lin X, et al. (2015) Enhanced leavening ability of baker's yeast by overexpression of snr84 with pgm2 deletion. J Ind Microbiol Biotechnol 42 (6): 939-948.Garcia Sanchez R, et al. (2010) Pgm2 overexpression improves anaerobic galactose fermentation in saccharomyces cerevisiae. Microb Cell Fact 9 40.Garcia Sanchez R, et al. (2010) Cross-reactions between engineered xylose and galactose pathways in recombinant saccharomyces cerevisiae. Biotechnol Biofuels 3 19.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,724 Da
NCBI Official Full Name
phosphoglucomutase-2
NCBI Official Synonym Full Names
phosphoglucomutase 2
NCBI Official Symbol
PGM2
NCBI Official Synonym Symbols
MSTP006
NCBI Protein Information
phosphoglucomutase-2
UniProt Protein Name
Phosphoglucomutase-2
UniProt Gene Name
PGM2
UniProt Synonym Gene Names
PGM 2
UniProt Entry Name
PGM2_HUMAN
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Product Notes
The PGM2 pgm2 (Catalog #AAA255179) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PGM2/phosphoglucomutase 2 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's PGM2/phosphoglucomutase 2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PGM2 pgm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGM2/phosphoglucomutase 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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