WB (Western Blot)
(Detection of human PHAX by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-PHAX antibody AAA212372 (lot AAA212372-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse PHAX Polyclonal Antibody | anti-PHAX antibody
Rabbit anti-PHAX Antibody, Affinity Purified
Gene Names
PHAX; RNUXA
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PHAX, Antibody; Rabbit anti-PHAX Antibody, Affinity Purified; phosphorylated adapter RNA export protein; RNA U small nuclear RNA export adapter protein; RNA U, small nuclear RNA export adapter (phosphorylation regulated); RNA U, small nuclear RNA export adaptor (phosphorylation regulated); RNUXA; Phosphorylated adapter RNA export protein; phosphorylated adaptor for RNA export; anti-PHAX antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PHAX antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 344 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PHAX by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-PHAX antibody AAA212372 (lot AAA212372-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human PHAX by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-PHAX antibody AAA212372 (lot AAA212372-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of mouse PHAX by western blot. Samples: Whole cell lysate from mouse NIH 3T3 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-PHAX antibody AAA212372 (lot AAA212372-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse PHAX by western blot. Samples: Whole cell lysate from mouse NIH 3T3 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-PHAX antibody AAA212372 (lot AAA212372-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PHAX by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PHAX antibody AAA212372 (lot AAA212372-1) used for IP at 6 ug/mg lysate. PHAX was also immunoprecipitated by rabbit anti-PHAX antibody BL14107. For blotting immunoprecipitated PHAX, AAA212372 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PHAX by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PHAX antibody AAA212372 (lot AAA212372-1) used for IP at 6 ug/mg lysate. PHAX was also immunoprecipitated by rabbit anti-PHAX antibody BL14107. For blotting immunoprecipitated PHAX, AAA212372 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-PHAX antibody
Phosphorylated adapter RNA export protein (PHAX) is a phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. PHAX also plays a role in the biogenesis of U3 small nucleolar RNA (snoRNA). It is involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H814].
Product Categories/Family for anti-PHAX antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,403 Da
NCBI Official Full Name
phosphorylated adapter RNA export protein
NCBI Official Synonym Full Names
phosphorylated adaptor for RNA export
NCBI Official Symbol
PHAX
NCBI Official Synonym Symbols
RNUXA
NCBI Protein Information
phosphorylated adapter RNA export protein; RNA U small nuclear RNA export adapter protein; RNA U, small nuclear RNA export adapter (phosphorylation regulated); RNA U, small nuclear RNA export adaptor (phosphorylation regulated)
UniProt Protein Name
Phosphorylated adapter RNA export protein
UniProt Gene Name
PHAX
UniProt Synonym Gene Names
RNUXA
UniProt Entry Name
PHAX_HUMAN
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Product Notes
The PHAX phax (Catalog #AAA212372) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PHAX Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PHAX can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PHAX phax for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHAX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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