WB (Western Blot)
(Detection of human PHD2 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHD2 antibody AAA210886 lot 5 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human PHD2 Polyclonal Antibody | anti-PHD2 antibody
Rabbit anti-PHD2 Antibody, Affinity Purified
Gene Names
EGLN1; HPH2; PHD2; SM20; ECYT3; HPH-2; HIFPH2; ZMYND6; C1orf12; HIF-PH2
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PHD2, Antibody; Rabbit anti-PHD2 Antibody, Affinity Purified; zinc finger MYND domain-containing protein 6; hypoxia-inducible factor prolyl hydroxylase 2; ZMYND6; PHD2; HALAH; prolyl hydroxylase domain-containing protein 2; HPH-2; SM-20; SM20; HIF-prolyl hydroxylase 2; HIFPH2; egl nine-like protein 1; egl nine homolog 1; ECYT3; C1orf12; HIF-PH2; HPH2; Egl nine homolog 1; egl-9 family hypoxia inducible factor 1; anti-PHD2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PHD2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PHD2 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHD2 antibody AAA210886 lot 5 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human PHD2 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, Hep-G2, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHD2 antibody AAA210886 lot 5 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human PHD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PHD2 antibody AAA210886 lot 5 used for IP at 6 ug per reaction. PHD2 was also immunoprecipitated by a previous lot of this antibody (AAA210886 lot 4) and another rabbit anti-PHD2 antibody (BL522). For blotting immunoprecipitated PHD2, AAA210886 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PHD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PHD2 antibody AAA210886 lot 5 used for IP at 6 ug per reaction. PHD2 was also immunoprecipitated by a previous lot of this antibody (AAA210886 lot 4) and another rabbit anti-PHD2 antibody (BL522). For blotting immunoprecipitated PHD2, AAA210886 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-PHD2 antibody
PHD2 is one of 4 PHD (PHD1-4) enzymes that function as oxygen sensors and are responsible for the post-translational modification of HIF-1alpha, a component of a transcriptional complex involved in oxygen homeostasis. During normoxic levels, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha and target it for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. The PHD isoforms appear to function in a non-redundant manner and may differ in their expression patterns and their catalytic selectivity.
Product Categories/Family for anti-PHD2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
426
NCBI Official Full Name
egl nine homolog 1
NCBI Official Synonym Full Names
egl-9 family hypoxia-inducible factor 1
NCBI Official Symbol
EGLN1
NCBI Official Synonym Symbols
HPH2; PHD2; SM20; ECYT3; HPH-2; HIFPH2; ZMYND6; C1orf12; HIF-PH2
NCBI Protein Information
egl nine homolog 1; egl nine-like protein 1; HIF prolyl hydroxylase 2; HIF-prolyl hydroxylase 2; zinc finger MYND domain-containing protein 6; hypoxia-inducible factor prolyl hydroxylase 2; prolyl hydroxylase domain-containing protein 2
UniProt Protein Name
Egl nine homolog 1
UniProt Gene Name
EGLN1
UniProt Synonym Gene Names
HIF-PH2; HIF-prolyl hydroxylase 2; HPH-2; PHD2
UniProt Entry Name
EGLN1_HUMAN
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Product Notes
The PHD2 egln1 (Catalog #AAA210886) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PHD2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHD2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PHD2 egln1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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