FCM/FACS (Flow Cytometry)
(PHGDH Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit PHGDH Polyclonal Antibody | anti-PHGDH antibody
PHGDH Antibody (Center)
Gene Names
PHGDH; NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113
Reactivity
Human (Predicted Reactivity: Monkey, Mouse, Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PHGDH, Antibody; PHGDH Antibody (Center); D-3-phosphoglycerate dehydrogenase; 3-PGDH; PHGDH; PGDH3; anti-PHGDH antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Monkey, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PHGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-277 amino acids from the Central region of human PHGDH.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
249-277
Sequence Length
533
Applicable Applications for anti-PHGDH antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(PHGDH Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PHGDH Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(PHGDH Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHGDH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(PHGDH Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHGDH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(PHGDH Antibody (Center) western blot analysis in Ramos cell line lysates (15ug/lane).This demonstrates the PHGDH antibody detected the PHGDH protein (arrow).)
WB (Western Blot)
(PHGDH Antibody (Center) western blot analysis in Ramos cell line lysates (15ug/lane).This demonstrates the PHGDH antibody detected the PHGDH protein (arrow).)
Related Product Information for anti-PHGDH antibody
3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95)
catalyzes the transition of 3-phosphoglycerate into
3-phosphohydroxypyruvate, which is the first and rate-limiting step
in the phosphorylated pathway of serine biosynthesis, using
NAD+/NADH as a cofactor.
catalyzes the transition of 3-phosphoglycerate into
3-phosphohydroxypyruvate, which is the first and rate-limiting step
in the phosphorylated pathway of serine biosynthesis, using
NAD+/NADH as a cofactor.
Product Categories/Family for anti-PHGDH antibody
References
Du, H., et al. Reproduction 139(1):237-245(2010)
Burton, R.L., et al. Biochemistry 48(22):4808-4815(2009)
Kim, J.W., et al. Psychiatr. Genet. 19 (3), 161 (2009) :
Tabatabaie, L., et al. Hum. Mutat. 30(5):749-756(2009)
Burton, R.L., et al. Biochemistry 48(22):4808-4815(2009)
Kim, J.W., et al. Psychiatr. Genet. 19 (3), 161 (2009) :
Tabatabaie, L., et al. Hum. Mutat. 30(5):749-756(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56651
NCBI Official Full Name
D-3-phosphoglycerate dehydrogenase
NCBI Official Synonym Full Names
phosphoglycerate dehydrogenase
NCBI Official Symbol
PHGDH
NCBI Official Synonym Symbols
NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113
NCBI Protein Information
D-3-phosphoglycerate dehydrogenase
UniProt Protein Name
D-3-phosphoglycerate dehydrogenase
UniProt Gene Name
PHGDH
UniProt Synonym Gene Names
PGDH3; 3-PGDH
UniProt Entry Name
SERA_HUMAN
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Product Notes
The PHGDH phgdh (Catalog #AAA285927) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 249-277. The PHGDH Antibody (Center) reacts with Human (Predicted Reactivity: Monkey, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PHGDH can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PHGDH phgdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHGDH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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