IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit Phospho-EP300 (S89) Polyclonal Antibody | anti-EP300 antibody
Phospho-EP300 (S89) Antibody
Gene Names
EP300; p300; KAT3B; RSTS2
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Dot Blot, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-EP300 (S89), Antibody; Phospho-EP300 (S89) Antibody; Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300; EP300; P300; anti-EP300 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EP300 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S89 of human EP300.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.45 (varies by lot)
Sequence Length
2414
Applicable Applications for anti-EP300 antibody
DB (Dot Blot), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
DB (Dot Blot)
(Dot blot analysis of anti-Phospho-EP300-S89 Pab on nitrocellulose membrane. 50ng of Phospho-peptide (BP3197a) or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.)
DB (Dot Blot)
(Dot blot analysis of anti-Phospho-EP300-S89 Pab on nitrocellulose membrane. 50ng of Phospho-peptide (BP3197a) or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.)
Related Product Information for anti-EP300 antibody
EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.
Product Categories/Family for anti-EP300 antibody
References
Finlan, L., et al., J. Biol. Chem. 279(47):49395-49405 (2004).
Dornan, D., et al., Mol. Cell. Biol. 24(22):10083-10098 (2004).
Jin, Y.H., et al., J. Biol. Chem. 279(28):29409-29417 (2004).
Kung, A.L., et al., Cancer Cell 6(1):33-43 (2004).
Chen, J., et al., Cell. Mol. Life Sci. 61(13):1675-1683 (2004).
Dornan, D., et al., Mol. Cell. Biol. 24(22):10083-10098 (2004).
Jin, Y.H., et al., J. Biol. Chem. 279(28):29409-29417 (2004).
Kung, A.L., et al., Cancer Cell 6(1):33-43 (2004).
Chen, J., et al., Cell. Mol. Life Sci. 61(13):1675-1683 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
264161
NCBI Official Full Name
histone acetyltransferase p300
NCBI Official Synonym Full Names
E1A binding protein p300
NCBI Official Symbol
EP300
NCBI Official Synonym Symbols
p300; KAT3B; RSTS2
NCBI Protein Information
histone acetyltransferase p300
UniProt Protein Name
Histone acetyltransferase p300
UniProt Gene Name
EP300
UniProt Synonym Gene Names
P300; p300 HAT
UniProt Entry Name
EP300_HUMAN
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Product Notes
The EP300 ep300 (Catalog #AAA285737) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-EP300 (S89) Antibody reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-EP300 (S89) can be used in a range of immunoassay formats including, but not limited to, DB (Dot Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the EP300 ep300 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-EP300 (S89), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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