DB (Dot Blot)
(Dot blot analysis of anti-Phospho-LIN28-S134 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Rabbit anti-Human Phospho-LIN28 (S134) Polyclonal Antibody | anti-LIN28A antibody
Phospho-LIN28 (S134) Antibody
Gene Names
LIN28A; CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
Reactivity
Human
Applications
ELISA, Dot Blot, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-LIN28 (S134), Antibody; Phospho-LIN28 (S134) Antibody; Protein lin-28 homolog A; Lin-28A; Zinc finger CCHC domain-containing protein 1; LIN28A; CSDD1; LIN28; ZCCHC1; anti-LIN28A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This LIN28 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S134 of human LIN28.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
209
Applicable Applications for anti-LIN28A antibody
ELISA, DB (Dot Blot), IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
DB (Dot Blot)
(Dot blot analysis of anti-Phospho-LIN28-S134 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
DB (Dot Blot)
(Dot blot analysis of anti-Phospho-LIN28-S134 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
IF (Immunofluorescence)
(Fluorescent confocal image of SY5Y cells stained with phospho- LIN28- S134 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA289649 phospho- LIN28- S134 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho- LIN28 immunosignal mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000131914).)
IF (Immunofluorescence)
(Fluorescent confocal image of SY5Y cells stained with phospho- LIN28- S134 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA289649 phospho- LIN28- S134 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho- LIN28 immunosignal mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000131914).)
Related Product Information for anti-LIN28A antibody
LIN28 is probable transcription factor. It plays a critical role in the control of immune response.
Product Categories/Family for anti-LIN28A antibody
References
References for protein:
1.Qiu, C., et al. Nucleic Acids Res. 38(4):1240-1248(2010)
2.Iliopoulos, D., et al. Cell 139(4):693-706(2009)
3.Heo, I., et al. Cell 138(4):696-708(2009)
References for SY5Y (SH-SY5Y; #CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
1.Qiu, C., et al. Nucleic Acids Res. 38(4):1240-1248(2010)
2.Iliopoulos, D., et al. Cell 139(4):693-706(2009)
3.Heo, I., et al. Cell 138(4):696-708(2009)
References for SY5Y (SH-SY5Y; #CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22743
NCBI Official Full Name
protein lin-28 homolog A
NCBI Official Synonym Full Names
lin-28 homolog A (C. elegans)
NCBI Official Symbol
LIN28A
NCBI Official Synonym Symbols
CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
NCBI Protein Information
protein lin-28 homolog A
UniProt Protein Name
Protein lin-28 homolog A
UniProt Gene Name
LIN28A
UniProt Synonym Gene Names
CSDD1; LIN28; ZCCHC1; Lin-28A
UniProt Entry Name
LN28A_HUMAN
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Product Notes
The LIN28A lin28a (Catalog #AAA289649) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-LIN28 (S134) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-LIN28 (S134) can be used in a range of immunoassay formats including, but not limited to, ELISA, DB (Dot Blot), IF (Immunofluorescence). Researchers should empirically determine the suitability of the LIN28A lin28a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-LIN28 (S134), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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