Rabbit Phospho-MAP4K1 (S171) Polyclonal Antibody | anti-MAP4K1 antibody

Phospho-MAP4K1 (S171) Antibody

Cat. Num. AAA28649

• Gene Names: MAP4K1; HPK1
• Reactivity: Human (Predicted: Mouse)
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Phospho-MAP4K1 (S171), Antibody; Phospho-MAP4K1 (S171) Antibody; Mitogen-activated protein kinase kinase kinase kinase 1; Hematopoietic progenitor kinase; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; MAP4K1; HPK1; anti-MAP4K1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This MAP4K1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S171 of human MAP4K1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

• Applicable Applications for anti-MAP4K1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Function: Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate.
• Tissue Location: Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
• Clone Names: RB12751
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of lysates from 293 cell line, untreated or treated with TPA, 200nM, 30min, using MAP4K1-pS171 (upper) or Tubulin (lower).)

Related Product Information for anti-MAP4K1 antibody

The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of blood cell differentiation. We have cloned a novel protein kinase, termed hematopoietic progenitor kinase 1 (HPK1), that is expressed predominantly in hematopoietic cells, including early progenitor cells. HPK1 is related distantly to the p21(Cdc42/Rac1)-activated kinase (PAK) and yeast STE20 implicated in the mitogen-activated protein kinase (MAPK) cascade. Expression of HPK1 activates JNK1 specifically, and it elevates strongly AP-1-mediated transcriptional activity in vivo. HPK1 binds and phosphorylates MEKK1 directly, whereas JNK1 activation by HPK1 is inhibited by a dominant-negative MEKK1 or MKK4/SEK mutant. Interestingly, unlike PAK65, HPK1 does not contain the small GTPase Rac1/Cdc42-binding domain and does not bind to either Rac1 or Cdc42, suggesting that HPK1. activation is Rac1/Cdc42-independent. These results indicate that HPK1 is a novel functional activator of the JNK/SAPK signaling pathway.

Product Categories/Family for anti-MAP4K1 antibody

Signal Transduction; Phospho Antibodies

References

Hu M.C.-T., Genes Dev. 10:2251-2264(1996).
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Wissing J., Mol. Cell. Proteomics 6:537-547(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 110611908
• NCBI GeneID: 11184
• NCBI Accession #: NP_001036065.1; NP_009112.1
• NCBI GenBank Nucleotide #: NM_001042600.2
• UniProt Accession #: Q92918
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 1 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 1
• NCBI Official Symbol: MAP4K1
• NCBI Official Synonym Symbols: HPK1
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 1
• UniProt Gene Name: MAP4K1
• UniProt Synonym Gene Names: HPK1; MEK kinase kinase 1; MEKKK 1
• UniProt Entry Name: M4K1_HUMAN