DB (Dot Blot)
(Dot blot analysis of Phospho-NR4A1-S351 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.)
Rabbit Phospho-NR4A1 (S351) Polyclonal Antibody | anti-NR4A1 antibody
Phospho-NR4A1 (S351) Antibody
Gene Names
NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
ELISA, Dot Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-NR4A1 (S351), Antibody; Phospho-NR4A1 (S351) Antibody; Nuclear receptor subfamily 4 group A member 1; Early response protein NAK1; Nuclear hormone receptor NUR/77; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; ST-59; Testicular receptor 3; NR4A1; GFRP1; HMR; NAK1; anti-NR4A1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NR4A1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S351 of human NR4A1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
325
Applicable Applications for anti-NR4A1 antibody
ELISA, DB (Dot Blot)
Antigen Type
Synthetic Peptide
Clone Names
RB11095
Antigen Source
HUMAN
Function
Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'- AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation.
Cellular Location
Cytoplasm. Nucleus.
Tissue Location
Fetal muscle and adult liver, brain and thyroid
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
DB (Dot Blot)
(Dot blot analysis of Phospho-NR4A1-S351 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.)
DB (Dot Blot)
(Dot blot analysis of Phospho-NR4A1-S351 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.)
Related Product Information for anti-NR4A1 antibody
NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis.
Product Categories/Family for anti-NR4A1 antibody
References
Lu, L., et al., J. Clin. Endocrinol. Metab. 89(8):4113-4118 (2004).
Castro-Obregon, S., et al., J. Biol. Chem. 279(17):17543-17553 (2004).
Lin, B., et al., Cell 116(4):527-540 (2004).
Choi, J.W., et al., Cancer Res. 64(1):35-39 (2004).
Ye, X., et al., Int. J. Biochem. Cell Biol. 36(1):98-113 (2004).
Castro-Obregon, S., et al., J. Biol. Chem. 279(17):17543-17553 (2004).
Lin, B., et al., Cell 116(4):527-540 (2004).
Choi, J.W., et al., Cancer Res. 64(1):35-39 (2004).
Ye, X., et al., Int. J. Biochem. Cell Biol. 36(1):98-113 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64463
NCBI Official Full Name
nuclear receptor subfamily 4 group A member 1 isoform 2
NCBI Official Synonym Full Names
nuclear receptor subfamily 4, group A, member 1
NCBI Official Symbol
NR4A1
NCBI Official Synonym Symbols
HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
NCBI Protein Information
nuclear receptor subfamily 4 group A member 1
UniProt Protein Name
Nuclear receptor subfamily 4 group A member 1
UniProt Gene Name
NR4A1
UniProt Synonym Gene Names
GFRP1; HMR; NAK1; Nur77
UniProt Entry Name
NR4A1_HUMAN
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Product Notes
The NR4A1 nr4a1 (Catalog #AAA287157) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-NR4A1 (S351) Antibody reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-NR4A1 (S351) can be used in a range of immunoassay formats including, but not limited to, ELISA, DB (Dot Blot). Researchers should empirically determine the suitability of the NR4A1 nr4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-NR4A1 (S351), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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