WB (Western Blot)
(Western blot using anti-mouse SphK1 (pS225) on mouse B-cell lysate. Antibody used at 1 ug/ml with phosphorylated blocking peptide (lane A) and without (laneB).)
Rabbit phospho-Sphingosine Kinase 1,(pS225), Mouse Reactive Polyclonal Antibody | anti-Sphk1 antibody
phospho-Sphingosine Kinase 1,(pS225), Mouse Reactive
Gene Names
Sphk1; Sk1; Spk1; 1110006G24Rik
Reactivity
Mouse
Applications
Western Blot
Purity
Purity: Affinity PurifiedPurification: Antigen Immunoaffiinity Purification
Synonyms
phospho-Sphingosine Kinase 1,(pS225), Mouse Reactive, Antibody; phospho-Sphingosine Kinase 1,(pS225), Mouse Reactive; phospho-Sphingosine Kinase 1,(pS225), Mouse Reactive. Rabbit Antigen Immunoaffinity Purified Polyclonal; anti-Sphk1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purity: Affinity Purified
Purification: Antigen Immunoaffiinity Purification
Purification: Antigen Immunoaffiinity Purification
Form/Format
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Concentration
100ug; 0.5mg/ml (varies by lot)
Sequence Length
382
Applicable Applications for anti-Sphk1 antibody
WB (Western Blot)
Immunogen
A synthetic peptide incorporating the serine 225 phosphorylation site of mouse sphingosie kinase 1
Comment
Antibody can be used for Western Blotting (1-5ug/ml). Antibody shows some cross reactivity (~10% by ELSIA) with other phosphoserine containing proteins. Optimal concentration should be evalutated by serial dilutions.
Preparation and Storage
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles
Stability: Products are stable for one year from purchase when stored properly.
Shipping: Ships on gel ice, store at -20ºC immediately upon arrival.
Stability: Products are stable for one year from purchase when stored properly.
Shipping: Ships on gel ice, store at -20ºC immediately upon arrival.
WB (Western Blot)
(Western blot using anti-mouse SphK1 (pS225) on mouse B-cell lysate. Antibody used at 1 ug/ml with phosphorylated blocking peptide (lane A) and without (laneB).)
WB (Western Blot)
(Western blot using anti-mouse SphK1 (pS225) on mouse B-cell lysate. Antibody used at 1 ug/ml with phosphorylated blocking peptide (lane A) and without (laneB).)
Related Product Information for anti-Sphk1 antibody
Activation of Sphingosine kinase (Sphk) increases the bioactive lipid, sphingosine 1-phosphate (S1P) and has been observed in a variety of cancer cells. Phosphorylation of sphingosine by sphingosine kinase (Sphk) is the rate-limiting step in the cellular synthesis of S1P. A principle activation event is the phosphorylation of serine 225 of sphiongosine kinase 1. Inhibition of Sphk1 phosphorylation and hence, Sphk activity is an important target for the development of drugs for cancer, inflammation, cardioprotection as well as a many other indications.
Product Categories/Family for anti-Sphk1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42.5
NCBI Official Full Name
sphingosine kinase 1 isoform 2
NCBI Official Synonym Full Names
sphingosine kinase 1
NCBI Official Symbol
Sphk1
NCBI Official Synonym Symbols
Sk1; Spk1; 1110006G24Rik
NCBI Protein Information
sphingosine kinase 1; SK 1; SPK 1
UniProt Protein Name
Sphingosine kinase 1
UniProt Gene Name
Sphk1
UniProt Synonym Gene Names
SK 1; SPK 1
UniProt Entry Name
SPHK1_MOUSE
Similar Products
Product Notes
The Sphk1 sphk1 (Catalog #AAA60497) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The phospho-Sphingosine Kinase 1,(pS225), Mouse Reactive reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's phospho-Sphingosine Kinase 1,(pS225), Mouse Reactive can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the Sphk1 sphk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "phospho-Sphingosine Kinase 1,(pS225), Mouse Reactive, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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