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IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse liver using Phospho-STAT5-Y694 antibody at dilution of 1:200 (40x lens).)
Rabbit Phospho-STAT5-Y694 Polyclonal Antibody | anti-STAT5-Y694 antibody
Phospho-STAT5-Y694 Rabbit pAb
Gene Names
STAT5A; MGF; STAT5
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity purification
Synonyms
Phospho-STAT5-Y694, Antibody; Phospho-STAT5-Y694 Rabbit pAb; MGF; STAT5; stat5a/stat5b; anti-STAT5-Y694 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Applicable Applications for anti-STAT5-Y694 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A phospho specific peptide corresponding to residues surrounding Y694 of human STAT5.
Positive Samples
K562
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse liver using Phospho-STAT5-Y694 antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse liver using Phospho-STAT5-Y694 antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human placenta using Phospho-STAT5-Y694 antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human placenta using Phospho-STAT5-Y694 antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded rat heart using Phospho-STAT5-Y694 antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded rat heart using Phospho-STAT5-Y694 antibody at dilution of 1:200 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of A-431 cells, using Phospho-STAT5-Y694 pAb at 1:1000 dilution or STAT5B antibody (A12356).A-431 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of A-431 cells, using Phospho-STAT5-Y694 pAb at 1:1000 dilution or STAT5B antibody (A12356).A-431 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of K562 cells, using Phospho-STAT5-Y694 pAb at 1:1000 dilution.K-562 cells were treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts of K562 cells, using Phospho-STAT5-Y694 pAb at 1:1000 dilution.K-562 cells were treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Related Product Information for anti-STAT5-Y694 antibody
Background: The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene.
Product Categories/Family for anti-STAT5-Y694 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90,647 Da
NCBI Official Full Name
signal transducer and activator of transcription 5A
NCBI Official Synonym Full Names
signal transducer and activator of transcription 5A
NCBI Official Symbol
STAT5A
NCBI Official Synonym Symbols
MGF; STAT5
NCBI Protein Information
signal transducer and activator of transcription 5A
UniProt Protein Name
Signal transducer and activator of transcription 5A
UniProt Gene Name
STAT5A
UniProt Synonym Gene Names
STAT5
UniProt Entry Name
STA5A_HUMAN
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Product Notes
The STAT5-Y694 stat5a (Catalog #AAA282113) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-STAT5-Y694 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-STAT5-Y694 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the STAT5-Y694 stat5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-STAT5-Y694, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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