IHC (Immunohiostchemistry)
(AAA329026 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit Phospho1 Polyclonal Antibody | anti-PHOSPHO1 antibody
Phospho1 Antibody
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Phospho1, Antibody; Phospho1 Antibody; PHOP1_HUMAN; Phosphatase orphan 1; Phospho 1; phospho1; Phosphoethanolamine/phosphocholine phosphatase; anti-PHOSPHO1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho1 Antibody detects endogenous levels of total Phospho1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid.
Concentration
1mg/ml (varies by lot)
Sequence Length
292
Applicable Applications for anti-PHOSPHO1 antibody
ELISA
Immunogen
A synthesized peptide derived from human Phospho1, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
IHC (Immunohiostchemistry)
(AAA329026 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA329026 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, using Phospho1 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, using Phospho1 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-PHOSPHO1 antibody
Function: Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization.
Tissue Specificity: Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize.
Similarity: Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.
Tissue Specificity: Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize.
Similarity: Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)29kD.
Predicted Molecular Weight: (Calculated)30kDa.
Predicted Molecular Weight: (Calculated)30kDa.
NCBI Official Full Name
phosphoethanolamine/phosphocholine phosphatase isoform 1
NCBI Official Synonym Full Names
phosphoethanolamine/phosphocholine phosphatase 1
NCBI Official Symbol
PHOSPHO1
NCBI Protein Information
phosphoethanolamine/phosphocholine phosphatase
UniProt Protein Name
Phosphoethanolamine/phosphocholine phosphatase
UniProt Gene Name
PHOSPHO1
UniProt Entry Name
PHOP1_HUMAN
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Product Notes
The PHOSPHO1 phospho1 (Catalog #AAA329026) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho1 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho1 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the PHOSPHO1 phospho1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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