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product-image-AAA133049_IHC11.jpg IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Mouse Tissue))

Rabbit anti-Mouse Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) Polyclonal Antibody | anti-PCK1 antibody

Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)

Gene Names
Pck1; PEPCK; Pck-1; AI265463
Reactivity
Mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Chromatography
Synonyms
Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1), Antibody; Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1); anti-PCK1 antibody
Ordering
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PCK1. It has been selected for its ability to recognize PCK1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-IAWMKFDAQ GNLRAINPEN GFFGVAPGTS VKTNPNAIKT IQKNTIFTNV AETSDGGVYW EGIDEPLAPG VTITSWKNKE WRPQDAEPCA HPNSRFCTPA SQCPIIDPAW ESPEGVPIEG IIFGGRRPEG VPLVYEALSW QHGVFVGAAM RSEATAAAEH KGKIIMHDPF AMRPFFGYNF GKYLAHWLSM AHRPAAKLPK IFHVNWFRKD KDGKFLWPGF GENSRVLEWM FGRIEGEDSA KLTPIGYIPK ENA
Applicable Applications for anti-PCK1 antibody
WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry)
Immunogen
Recombinant PCK1 (Ile312~Ala563) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

IHC (Immunohistochemisry)

(DAB staining on IHC-P. Samples: Mouse Tissue))

product-image-AAA133049_IHC11.jpg IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Mouse Tissue))

WB (Western Blot)

(Western Blot: Sample: Recombinant PCK1, Mouse.)

product-image-AAA133049_WB13.jpg WB (Western Blot) (Western Blot: Sample: Recombinant PCK1, Mouse.)

WB (Western Blot)

(Western Blot: Sample: Lane1: Mouse Liver Tissue; Lane2: Rat Liver Tissue; Lane3: Mouse Kidney Tissue.)

product-image-AAA133049_WB15.jpg WB (Western Blot) (Western Blot: Sample: Lane1: Mouse Liver Tissue; Lane2: Rat Liver Tissue; Lane3: Mouse Kidney Tissue.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
69,355 Da
NCBI Official Full Name
phosphoenolpyruvate carboxykinase, cytosolic
NCBI Official Synonym Full Names
phosphoenolpyruvate carboxykinase 1, cytosolic
NCBI Official Symbol
Pck1
NCBI Official Synonym Symbols
PEPCK; Pck-1; AI265463
NCBI Protein Information
phosphoenolpyruvate carboxykinase, cytosolic [GTP]
UniProt Protein Name
Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
UniProt Gene Name
Pck1
UniProt Synonym Gene Names
PEPCK-C

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Product Notes

The PCK1 pck1 (Catalog #AAA133049) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the PCK1 pck1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-IAW MKFDAQ GNLRAINPEN GFFGVAPGTS VKTNPNAIKT IQKNTIFTNV AETSDGGVYW EGIDEPLAPG VTITSWKNKE WRPQDAEPCA HPNSRFCTPA SQCPIIDPAW ESPEGVPIEG IIFGGRRPEG VPLVYEALSW QHGVFVGAAM RSEATAAAEH KGKIIMHDPF AMRPFFGYNF GKYLAHWLSM AHRPAAKLPK IFHVNWFRKD KDGKFLWPGF GENSRVLEWM FGRIEGEDSA KLTPIGYIPK ENA. It is sometimes possible for the material contained within the vial of "Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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