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IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.))
Rabbit Phospholipase A2, Calcium Independent (iPLA2) Polyclonal Antibody | anti-iPLA2 antibody
Polyclonal Antibody to Phospholipase A2, Calcium Independent (iPLA2)
Gene Names
Pla2g6; iPLA2; PNPLA9; BB112799; iPLA2beta; iPLA(2)beta
Reactivity
Mouse, Human, Rat
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Phospholipase A2, Calcium Independent (iPLA2), Antibody; Polyclonal Antibody to Phospholipase A2, Calcium Independent (iPLA2); anti-iPLA2 antibody
Host
Rabbit
Reactivity
Mouse, Human, Rat
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
0.22mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LLCLDGGGVK GLVIIQLLIA IEKASGVATK DLFDWVAGTS TGGILALAIL HSKSMAYMRG VYFRMKDEVF RGSRPYESGP LEEFLKREFG EHTKMTDVKK PKVMLTGTLS DRQPAELHLF RNYDAPEAVR EPRCNQNINL KPPTQPADQL VWRAARSSGA APTYFRPNGR FLDGGLLANN PTLDAMTEIH EYNQDMIRKG
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LLCLDGGGVK GLVIIQLLIA IEKASGVATK DLFDWVAGTS TGGILALAIL HSKSMAYMRG VYFRMKDEVF RGSRPYESGP LEEFLKREFG EHTKMTDVKK PKVMLTGTLS DRQPAELHLF RNYDAPEAVR EPRCNQNINL KPPTQPADQL VWRAARSSGA APTYFRPNGR FLDGGLLANN PTLDAMTEIH EYNQDMIRKG
Applicable Applications for anti-iPLA2 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Mouse, Rat
Immunogen
Recombinant iPLA2 (Leu481~Val702 (Accession # P97819)) expressed in E.coli (Immunogen: Recombinant iPLA2 (Leu481~Val702 (Accession # P97819)) expressed
in E.coli ( MBS2018626)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.))
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.))
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
WB (Western Blot)
(Western Blot: Sample: Recombinant iPLA2, Mouse.)
WB (Western Blot)
(Western Blot: Sample: Recombinant iPLA2, Mouse.)
WB (Western Blot)
(Western Blot: Sample: Mouse Testis lysate;Primary Ab: 1ug/ml Rabbit Anti-Mouse iPLA2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Mouse Testis lysate;Primary Ab: 1ug/ml Rabbit Anti-Mouse iPLA2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
85/88 kDa calcium-independent phospholipase A2 isoform 2
NCBI Official Synonym Full Names
phospholipase A2, group VI
NCBI Official Symbol
Pla2g6
NCBI Official Synonym Symbols
iPLA2; PNPLA9; BB112799; iPLA2beta; iPLA(2)beta
NCBI Protein Information
85/88 kDa calcium-independent phospholipase A2
UniProt Protein Name
85/88 kDa calcium-independent phospholipase A2
UniProt Gene Name
Pla2g6
UniProt Synonym Gene Names
Pnpla9; CaI-PLA2; GVI PLA2; iPLA2-beta; PNPLA9
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Product Notes
The iPLA2 pla2g6 (Catalog #AAA134952) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Phospholipase A2, Calcium Independent (iPLA2) reacts with Mouse, Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phospholipase A2, Calcium Independent (iPLA2) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the iPLA2 pla2g6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LLCLDGGGVK GLVIIQLLIA IEKASGVATK DLFDWVAGTS TGGILALAIL HSKSMAYMRG VYFRMKDEVF RGSRPYESGP LEEFLKREFG EHTKMTDVKK PKVMLTGTLS DRQPAELHLF RNYDAPEAVR EPRCNQNINL KPPTQPADQL VWRAARSSGA APTYFRPNGR FLDGGLLANN PTLDAMTEIH EYNQDMIRKG. It is sometimes possible for the material contained within the vial of "Phospholipase A2, Calcium Independent (iPLA2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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