WB (Western Blot)
(PHOX2B Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the PHOX2B antibody detected the PHOX2B protein (arrow).)
Rabbit PHOX2B Polyclonal Antibody | anti-PHOX2B antibody
PHOX2B Antibody (Center)
Gene Names
PHOX2B; PMX2B; NBLST2; NBPhox
Reactivity
Mouse (Predicted Reactivity: Human, Rat)
Applications
Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PHOX2B, Antibody; PHOX2B Antibody (Center); Paired mesoderm homeobox protein 2B; Neuroblastoma Phox; NBPhox; PHOX2B homeodomain protein; Paired-like homeobox 2B; PHOX2B; PMX2B; anti-PHOX2B antibody
Host
Rabbit
Reactivity
Mouse (Predicted Reactivity: Human, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PHOX2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-130 amino acids from the Central region of human PHOX2B.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
104-130
Sequence Length
314
Applicable Applications for anti-PHOX2B antibody
WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Function
Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum- response element.
Cellular Location
Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108}
Tissue Location
Expressed in neuroblastoma, brain and adrenal gland.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(PHOX2B Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the PHOX2B antibody detected the PHOX2B protein (arrow).)
WB (Western Blot)
(PHOX2B Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the PHOX2B antibody detected the PHOX2B protein (arrow).)
IF (Immunofluorescence)
(Fluorescent image of U251 cell stained with PHOX2B Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PHOX2B primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).PHOX2B immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent image of U251 cell stained with PHOX2B Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PHOX2B primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).PHOX2B immunoreactivity is localized to Nucleus significantly.)
Related Product Information for anti-PHOX2B antibody
The DNA-associated protein encoded by this gene is a
member of the paired family of homeobox proteins localized to the
nucleus. The protein functions as a transcription factor involved
in the development of several major noradrenergic neuron
populations and the determination of neurotransmitter phenotype.
The gene product is linked to enhancement of second
messenger-mediated activation of the dopamine beta-hydroylase,
c-fos promoters and several enhancers, including cyclic
amp-response element and serum-response element. [provided by
RefSeq].
member of the paired family of homeobox proteins localized to the
nucleus. The protein functions as a transcription factor involved
in the development of several major noradrenergic neuron
populations and the determination of neurotransmitter phenotype.
The gene product is linked to enhancement of second
messenger-mediated activation of the dopamine beta-hydroylase,
c-fos promoters and several enhancers, including cyclic
amp-response element and serum-response element. [provided by
RefSeq].
Product Categories/Family for anti-PHOX2B antibody
References
Rudzinski, E., et al. Pediatr. Dev. Pathol. 13(4):291-299(2010)
Janoueix-Lerosey, I., et al. Oncogene 29(11):1566-1579(2010)
Tu, E., et al. Hum. Pathol. 41(3):392-400(2010)
Arai, H., et al. J. Hum. Genet. 55(1):4-7(2010)
Dubreuil, V., et al. J. Neurosci. 29(47):14836-14846(2009)
Janoueix-Lerosey, I., et al. Oncogene 29(11):1566-1579(2010)
Tu, E., et al. Hum. Pathol. 41(3):392-400(2010)
Arai, H., et al. J. Hum. Genet. 55(1):4-7(2010)
Dubreuil, V., et al. J. Neurosci. 29(47):14836-14846(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31621
NCBI Official Full Name
paired mesoderm homeobox protein 2B
NCBI Official Synonym Full Names
paired-like homeobox 2b
NCBI Official Symbol
PHOX2B
NCBI Official Synonym Symbols
PMX2B; NBLST2; NBPhox
NCBI Protein Information
paired mesoderm homeobox protein 2B
UniProt Protein Name
Paired mesoderm homeobox protein 2B
UniProt Gene Name
PHOX2B
UniProt Synonym Gene Names
PMX2B; NBPhox
UniProt Entry Name
PHX2B_HUMAN
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Product Notes
The PHOX2B phox2b (Catalog #AAA286193) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 104-130. The PHOX2B Antibody (Center) reacts with Mouse (Predicted Reactivity: Human, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PHOX2B can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the PHOX2B phox2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHOX2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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