Rabbit anti-Human PI4K2A (PI4K II) Polyclonal Antibody | anti-PI4K2A antibody

PI4K2A (PI4K II) Antibody (N-term)

Cat. Num. AAA289134

• Gene Names: PI4K2A; PI4KII; PIK42A
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PI4K2A (PI4K II), Antibody; PI4K2A (PI4K II) Antibody (N-term); Phosphatidylinositol 4-kinase type 2-alpha; Phosphatidylinositol 4-kinase type II-alpha; PI4K2A; anti-PI4K2A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This PI4K2A (PI4K II) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PI4K2A (PI4K II).
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 1-30
• Sequence Length: 479

• Applicable Applications for anti-PI4K2A antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Membrane-bound phosphatidylinositol-4 kinase (PI4-kinase) that catalyzes the phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P), a lipid that plays important roles in endocytosis, Golgi function, protein sorting and membrane trafficking and is required for prolonged survival of neurons. Besides, phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4- phosphate (PI4P) is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3).
• Cellular Location: Golgi apparatus, trans-Golgi network membrane; Lipid-anchor. Membrane raft. Cell projection, dendrite {ECO:0000250|UniProtKB:Q2TBE6}. Presynaptic cell membrane {ECO:0000250|UniProtKB:Q2TBE6}. Synapse, synaptosome {ECO:0000250|UniProtKB:Q2TBE6}. Mitochondrion {ECO:0000250|UniProtKB:Q2TBE6}. Endosome. Endosome membrane. Cytoplasmic vesicle. Membrane; Lipid-anchor. Cell membrane. Perikaryon {ECO:0000250|UniProtKB:Q2TBE6}. Cell projection, neuron projection {ECO:0000250|UniProtKB:Q2TBE6}. Note=Found in subdomains of the plasma membrane termed non-caveolar membrane rafts. Transported from neuronal cell body to neuron projections and neurite tips in a BLOC-1- and AP-3- complexes-dependent manner. {ECO:0000250|UniProtKB:Q2TBE6}
• Tissue Location: Widely expressed. Highest expression is observed in kidney, brain, heart, skeletal muscle, and placenta and lowest expression is observed in colon, thymus, and small intestine
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Negative control of hela cells without Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).Alexa-Fluor-546 emits orange fluorescence. Immunofluorescence analysis of PI4K2A (PI4K II) Antibody (N-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).)

IHC (Immunohistochemistry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(Anti-PI4K II Antibody (C-term) at 1:8000 dilution + human kidney lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.)

WB (Western Blot)

(Western blot analysis of anti-PI4K2A Antibody (N-term D2) (Cat.#please inquire) in mouse brain tissue lysates (35ug/lane). PI4KII(arrow) was detected using the purified Pab.)

WB (Western Blot)

(Western blot analysis of anti-PI4K II Pab in HL60 cell lysate. PI4K II (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Related Product Information for anti-PI4K2A antibody

Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs. PI4K II is activated by detergent and inhibited by adenosine. Overexpression of PI4KII in COS-7 cells increases synthesis of PtdIns4P. Some cells overexpressing PI4KII have scattered or no perinuclear Golgi. Knockdown of PI4KII by RNA interference (RNAi) does not disrupt the Golgi, and some cells show expanded Golgi. RNAi reduces the Golgi level of PtdIns4P and blocks the association between AP1 and the trans-Golgi network. PI4KII RNAi had little effect on intra-Golgi trafficking, but it inhibited export to plasma membrane export by 35%. It has been proposed that PI4KII generates PtdIns4P-rich domains within the Golgi that specify docking of the AP1 coat machinery.

Product Categories/Family for anti-PI4K2A antibody

Signal Transduction

References

Wang, Y.J., et al., Cell 114(3):299-310 (2003). Minogue, S., et al., J. Biol. Chem. 276(20):16635-16640 (2001). Barylko, B., et al., J. Biol. Chem. 276(11):7705-7708 (2001). Waugh, M.G., et al., Biochem. J. 373 (Pt 1), 57-63 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 13559514
• NCBI GeneID: 55361
• NCBI Accession #: NP_060895.1
• NCBI GenBank Nucleotide #: NM_018425.3
• UniProt Accession #: Q9BTU6; Q9NSG8; D3DR59
• Molecular Weight: 54022
• NCBI Official Full Name: phosphatidylinositol 4-kinase type 2-alpha
• NCBI Official Synonym Full Names: phosphatidylinositol 4-kinase type 2 alpha
• NCBI Official Symbol: PI4K2A
• NCBI Official Synonym Symbols: PI4KII; PIK42A
• NCBI Protein Information: phosphatidylinositol 4-kinase type 2-alpha
• UniProt Protein Name: Phosphatidylinositol 4-kinase type 2-alpha
• UniProt Gene Name: PI4K2A
• UniProt Entry Name: P4K2A_HUMAN